PMID- 6137109
OWN - NLM
STAT- MEDLINE
DCOM- 19831028
LR  - 20190919
IS  - 0340-6075 (Print)
IS  - 0340-6075 (Linking)
VI  - 43
IP  - 2
DP  - 1983
TI  - Effect of iron on the stability of macrophage lysosomes.
PG  - 85-101
AB  - Mouse peritoneal macrophages exposed to 60 microM Fe3+ in culture showed a 
      pronounced decline in survival rate and an enhanced capacity for lipid 
      peroxidation, as estimated by the formation of malondialdehyde. The iron-exposed 
      cells contain secondary lysosomes loaded with iron as demonstrated by 
      transmission electron microscopy, energy dispersive X-ray micro-analysis and the 
      sulphide-silver method. The macrophages concentrated within their lysosomes the 
      weak base acridine orange (AO) when the latter was added to the medium in a low 
      concentration (1:10(5)). When cells were viewed under continuous illumination 
      with short wave blue light in a fluorescence microscope, AO-exposed cells 
      initially showed a brilliant red granular fluorescence and subsequently a 
      progressive redistribution of AO into the cell sap and the nucleus with resultant 
      green fluorescence. This redistribution of AO was associated with the 
      disappearance of the normal granular reaction product pattern of the lysosomal 
      marker enzyme acid phosphatase (AP), as demonstrated with a Gomori-type reaction, 
      and later with cell death. The redistribution of AO and AP from the lysosomal 
      vacuome to the cell sap under the influence of blue light occurred markedly more 
      rapidly in macrophages containing iron loaded lysosomes than in control cells. 
      The findings indicate that the presence of iron in lysosomes decreases the 
      proton-retaining ability of their bordering membranes, possibly by way of 
      enhanced metal (iron) catalyzed lipid peroxidation. It appears that leakage of AO 
      signifies destabilization of the lysosomal membrane.
FAU - Abok, K
AU  - Abok K
FAU - Hirth, T
AU  - Hirth T
FAU - Ericsson, J L
AU  - Ericsson JL
FAU - Brunk, U
AU  - Brunk U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Virchows Arch B Cell Pathol Incl Mol Pathol
JT  - Virchows Archiv. B, Cell pathology including molecular pathology
JID - 9316922
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Animals
MH  - Cell Survival/drug effects
MH  - Female
MH  - Iron/*pharmacology
MH  - Lysosomes/*drug effects
MH  - Macrophages/*drug effects/ultrastructure
MH  - Male
MH  - Membranes/drug effects
MH  - Mice
MH  - Microscopy, Electron
EDAT- 1983/01/01 00:00
MHDA- 1983/01/01 00:01
CRDT- 1983/01/01 00:00
PHST- 1983/01/01 00:00 [pubmed]
PHST- 1983/01/01 00:01 [medline]
PHST- 1983/01/01 00:00 [entrez]
AID - 10.1007/BF02932947 [doi]
PST - ppublish
SO  - Virchows Arch B Cell Pathol Incl Mol Pathol. 1983;43(2):85-101. doi: 
      10.1007/BF02932947.