PMID- 6137561
OWN - NLM
STAT- MEDLINE
DCOM- 19831123
LR  - 20190511
IS  - 0022-3751 (Print)
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Linking)
VI  - 341
DP  - 1983 Aug
TI  - Blockade of amino acid-induced depolarizations and inhibition of excitatory 
      post-synaptic potentials in rat dentate gyrus.
PG  - 627-40
AB  - Excitatory post-synaptic potentials (e.p.s.p.s) evoked by stimulation of the 
      medial perforant path and depolarizations induced by excitatory amino acids were 
      recorded from granule cells in the preparation of the hippocampal slice from the 
      rat. The effects of (+/-)-2-amino-5-phosphonovalerate (APV), 
      gamma-D-glutamylglycine (gamma DGG) and cis-2,3-piperidinedicarboxylate (PDA), 
      antagonists of excitatory amino acids on these phenomena were compared. gamma DGG 
      was the most effective antagonist of the e.p.s.p. Its action was reversible and 
      not associated with any change in the passive membrane properties of the granule 
      cells or in the apparent reversal potential of the e.p.s.p. Quantal analysis 
      showed that the reduction in the e.p.s.p. paralleled the decrease in quantal size 
      rather than quantal content, confirming a post-synaptic site of the action of 
      gamma DGG. The potency of gamma DGG against the exogenous agonists was 
      N-methyl-D-aspartate greater than kainate greater than or equal to quisqualate. 
      APV had very little effect on the e.p.s.p. but was a selective antagonist of 
      N-methyl-D-aspartate-induced depolarizations. PDA depolarized granule cells and 
      increased their membrane input resistance. Although gamma DGG was a potent 
      antagonist of both glutamate- and aspartate-induced depolarizations, no clear 
      pattern of specificity could be found. The action of glutamate was unaffected by 
      APV. These results indicate that the receptor for the transmitter at the synapses 
      formed by the fibres of the perforant path with the granule cells is of the 
      quisqualate and/or kainate type. The present data are consistent with the 
      biochemical evidence that glutamate may be the endogenous transmitter at his 
      synapse.
FAU - Crunelli, V
AU  - Crunelli V
FAU - Forda, S
AU  - Forda S
FAU - Kelly, J S
AU  - Kelly JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (Amino Acids)
RN  - 0 (Dipeptides)
RN  - 0 (Oxadiazoles)
RN  - 0 (Pipecolic Acids)
RN  - 1948-29-4 (gamma-glutamylglycine)
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - 46026-75-9 (2,3-piperidinedicarboxylic acid)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - 76726-92-6 (2-Amino-5-phosphonovalerate)
RN  - 8OC22C1B99 (Quisqualic Acid)
RN  - HG18B9YRS7 (Valine)
RN  - SIV03811UC (Kainic Acid)
SB  - IM
MH  - 2-Amino-5-phosphonovalerate
MH  - Amino Acids/*pharmacology
MH  - Animals
MH  - Aspartic Acid/analogs & derivatives/antagonists & inhibitors
MH  - Dipeptides/pharmacology
MH  - Evoked Potentials/drug effects
MH  - Hippocampus/*physiology
MH  - In Vitro Techniques
MH  - Kainic Acid/pharmacology
MH  - Membrane Potentials/drug effects
MH  - N-Methylaspartate
MH  - Oxadiazoles/pharmacology
MH  - Pipecolic Acids/pharmacology
MH  - Quisqualic Acid
MH  - Rats
MH  - Synapses/physiology
MH  - Valine/analogs & derivatives/pharmacology
PMC - PMC1195354
EDAT- 1983/08/01 00:00
MHDA- 1983/08/01 00:01
PMCR- 1983/08/01
CRDT- 1983/08/01 00:00
PHST- 1983/08/01 00:00 [pubmed]
PHST- 1983/08/01 00:01 [medline]
PHST- 1983/08/01 00:00 [entrez]
PHST- 1983/08/01 00:00 [pmc-release]
AID - 10.1113/jphysiol.1983.sp014829 [doi]
PST - ppublish
SO  - J Physiol. 1983 Aug;341:627-40. doi: 10.1113/jphysiol.1983.sp014829.