PMID- 6138095
OWN - NLM
STAT- MEDLINE
DCOM- 19831220
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 22
IP  - 20
DP  - 1983 Sep 27
TI  - Taxol effect on tubulin polymerization and associated guanosine 5'-triphosphate 
      hydrolysis.
PG  - 4814-22
AB  - Taxol has been used as a tool to investigate the relationship between microtubule 
      assembly and guanosine 5'-triphosphate (GTP) hydrolysis. The data support the 
      model previously proposed [Carlier, M.-F., & Pantaloni, D. (1981) Biochemistry 
      20, 1918] that GTP hydrolysis is not tightly coupled to the polymerization 
      process but takes place as a monomolecular process following polymerization. The 
      results further indicate that the energy liberated by GTP hydrolysis is not 
      responsible for the subsequent blockage of GDP on polymerized tubulin. When 
      tubulin is polymerized in the presence of 10-100 microM taxol, the rapid 
      formation of a large number of very short microtubules (l less than 1 micron) is 
      accompanied by the development of turbidity to a lesser extent than what is 
      observed when the same weight amount of longer microtubules (l = 5 microns) is 
      formed. A slower subsequent turbidity increase corresponds to the length 
      redistribution of these short microtubules into 3-5-fold longer ones without any 
      change in the weight amount of polymer. The evolution of the rate of length 
      redistribution with the concentration of taxol suggests a model within which 
      taxol would bind to dimeric tubulin and to tubulin present at the ends of 
      microtubules with a somewhat 10-fold lower affinity than to polymerized tubulin 
      embedded in the bulk of microtubules. In agreement with this model, binding of 
      taxol to the tubulin-colchicine complex in the dimeric form could be measured 
      from the increase in the GTPase activity of the tubulin-colchicine complex 
      accompanying taxol binding.
FAU - Carlier, M F
AU  - Carlier MF
FAU - Pantaloni, D
AU  - Pantaloni D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Alkaloids)
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Tubulin)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - P88XT4IS4D (Paclitaxel)
RN  - SML2Y3J35T (Colchicine)
SB  - IM
MH  - Alkaloids/*pharmacology
MH  - Animals
MH  - Antineoplastic Agents, Phytogenic
MH  - Brain/metabolism
MH  - Colchicine/metabolism
MH  - Guanosine Triphosphate/*metabolism
MH  - Kinetics
MH  - Macromolecular Substances
MH  - Microtubules/ultrastructure
MH  - Paclitaxel
MH  - Swine
MH  - Tubulin/*metabolism
EDAT- 1983/09/27 00:00
MHDA- 1983/09/27 00:01
CRDT- 1983/09/27 00:00
PHST- 1983/09/27 00:00 [pubmed]
PHST- 1983/09/27 00:01 [medline]
PHST- 1983/09/27 00:00 [entrez]
AID - 10.1021/bi00289a031 [doi]
PST - ppublish
SO  - Biochemistry. 1983 Sep 27;22(20):4814-22. doi: 10.1021/bi00289a031.