PMID- 6139269
OWN - NLM
STAT- MEDLINE
DCOM- 19840107
LR  - 20131121
IS  - 0198-0238 (Print)
IS  - 0198-0238 (Linking)
VI  - 2
IP  - 3
DP  - 1983
TI  - Induction of the acute-phase reactant, alpha 1-acid glycoprotein, by 
      glucocorticoids in rat hepatoma cells.
PG  - 205-12
AB  - alpha 1-acid glycoprotein (alpha 1-AGP), or orosomucoid, is shown to be inducible 
      by glucocorticoids in HTC rat hepatoma cells. Immunoprecipitation of 
      [35S]methionine pulse-labeled proteins from these cells reveals secreted proteins 
      of Mr = 35,000-48,000 (alpha 1-AGP) and Mr greater than 180,000, both of which 
      are greatly enhanced by glucocorticoid treatment. The amount of alpha 
      1-AGP-specific mRNA in HTC cells is greatly increased (at least 100-fold) in 
      response to glucocorticoids. The new steady-state level of RNA is approached with 
      a t 1/2 of about 8 hr and the RNA consists of a single species of approximately 
      850 bases. The response is specific for glucocorticoids since: (i) the EC50 for 
      dexamethasone is 30 nM; (ii) the glucocorticoid antagonist, progesterone, 
      inhibits the induction by dexamethasone; and (iii) a glucocorticoid 
      receptor-deficient cell line is incapable of alpha 1-AGP mRNA induction. This is 
      a secondary hormonal response since inhibition of protein synthesis blocks the 
      induction of alpha 1-AGP mRNA by dexamethasone whereas the induction of mouse 
      mammary tumor virus (MMTV) RNA is unaffected.
FAU - Vannice, J L
AU  - Vannice JL
FAU - Ringold, G M
AU  - Ringold GM
FAU - McLean, J W
AU  - McLean JW
FAU - Taylor, J M
AU  - Taylor JM
LA  - eng
GR  - CA33563/CA/NCI NIH HHS/United States
GR  - GM07149/GM/NIGMS NIH HHS/United States
GR  - GM25821/GM/NIGMS NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - DNA
JT  - DNA (Mary Ann Liebert, Inc.)
JID - 8302432
RN  - 0 (Glucocorticoids)
RN  - 0 (Orosomucoid)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 98600C0908 (Cycloheximide)
RN  - EC 2.6.1.5 (Tyrosine Transaminase)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cycloheximide/pharmacology
MH  - Dexamethasone/*pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Glucocorticoids/pharmacology
MH  - Liver Neoplasms, Experimental
MH  - Orosomucoid/*biosynthesis
MH  - Progesterone/pharmacology
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Receptors, Glucocorticoid/metabolism
MH  - Tyrosine Transaminase/biosynthesis
MH  - Virus Activation/drug effects
EDAT- 1983/01/01 00:00
MHDA- 1983/01/01 00:01
CRDT- 1983/01/01 00:00
PHST- 1983/01/01 00:00 [pubmed]
PHST- 1983/01/01 00:01 [medline]
PHST- 1983/01/01 00:00 [entrez]
AID - 10.1089/dna.1983.2.205 [doi]
PST - ppublish
SO  - DNA. 1983;2(3):205-12. doi: 10.1089/dna.1983.2.205.