PMID- 6140673
OWN - NLM
STAT- MEDLINE
DCOM- 19840214
LR  - 20190818
IS  - 0196-9781 (Print)
IS  - 0196-9781 (Linking)
VI  - 4
IP  - 5
DP  - 1983 Sep-Oct
TI  - Characterization and localization of 3H-arginine8-vasopressin binding to rat 
      kidney and brain tissue.
PG  - 699-706
AB  - Anatomic, behavioral and pharmacologic evidence suggests that 
      arginine8-vasopressin (AVP) serves as a CNS neurotransmitter or neuromodulator. 
      We have characterized AVP binding to membrane and tissue slice preparations from 
      brain and kidney, and examined the anatomical distribution of these binding 
      sites. Conditions for the binding assay were optimized using kidney medullary 
      tissue. Binding of 3H-AVP (S.A. = 30-51 Ci/mmol, NEN) to brain and kidney 
      membranes and tissue slices was saturable, temperature dependent, linearly 
      related to protein concentration (or number of tissue slices), reversible, and 
      specific since the ability of cold AVP to displace 3H-AVP from binding was 
      greater than oxytocin and other related peptide fragments. Autoradiographic 
      localization of 3H-AVP binding was restricted to kidney medullary tissue. In 
      brain tissue, 3H-AVP binding was found to occur in concentrated foci. Brainstem 
      areas such as the nucleus tractus solitarius (NTS) showed a high density of AVP 
      binding sites. Since local injections of AVP into the NTS have been shown to 
      influence blood pressure, the present study presents the first anatomical 
      evidence for the presence of AVP specific binding sites which might mediate this 
      effect.
FAU - Dorsa, D M
AU  - Dorsa DM
FAU - Majumdar, L A
AU  - Majumdar LA
FAU - Petracca, F M
AU  - Petracca FM
FAU - Baskin, D G
AU  - Baskin DG
FAU - Cornett, L E
AU  - Cornett LE
LA  - eng
GR  - AM 17047/AM/NIADDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Peptides
JT  - Peptides
JID - 8008690
RN  - 0 (Neurotransmitter Agents)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Vasopressin)
RN  - 10028-17-8 (Tritium)
RN  - 113-79-1 (Arginine Vasopressin)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
SB  - IM
MH  - Adenylyl Cyclases
MH  - Animals
MH  - Arginine Vasopressin/*metabolism
MH  - Autoradiography
MH  - Brain/*metabolism
MH  - Brain Stem/metabolism
MH  - Cell Membrane/metabolism
MH  - In Vitro Techniques
MH  - Kidney/*metabolism
MH  - Kidney Medulla/metabolism
MH  - Kinetics
MH  - Neurotransmitter Agents
MH  - Rats
MH  - Receptors, Cell Surface/isolation & purification/*metabolism
MH  - *Receptors, Vasopressin
MH  - Tritium
EDAT- 1983/09/01 00:00
MHDA- 1983/09/01 00:01
CRDT- 1983/09/01 00:00
PHST- 1983/09/01 00:00 [pubmed]
PHST- 1983/09/01 00:01 [medline]
PHST- 1983/09/01 00:00 [entrez]
AID - 0196-9781(83)90021-9 [pii]
AID - 10.1016/0196-9781(83)90021-9 [doi]
PST - ppublish
SO  - Peptides. 1983 Sep-Oct;4(5):699-706. doi: 10.1016/0196-9781(83)90021-9.