PMID- 6155204
OWN - NLM
STAT- MEDLINE
DCOM- 19800815
LR  - 20190620
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 45
IP  - 9
DP  - 1980 May 1
TI  - Bladder cancer diagnosis by flow cytometry. Correlation between cell samples from 
      biopsy and bladder irrigation fluid.
PG  - 2389-94
AB  - Results of flow cytometry (FCM) examinations of bladder irrigation specimens were 
      compared with those of FCM examinations of cell suspensions from bladder biopsies 
      of 44 urologic patients. The fluorescent dye, acridine orange (AO), was used to 
      stain DNA and RNA differentially and abnormal urothelial cells were identified by 
      their relative content of nucleic acids. Granulocytes and squamous cells could be 
      distinguished from transitional cells in this procedure, and did not interfere 
      with the analyses. Of 28 patients with papillary carcinoma, carcinoma in situ, 
      and invasive carcinoma 27 were identified through FCM examination of irrigation 
      cytology specimens; the one false-negative result was from a low-grade papillary 
      carcinoma. Of 7 patients with papilloma, FCM examinations of irrigation specimens 
      were positive in 4 and negative in 3. Results of FCM studies of biopsy specimens 
      were in good but not complete agreement with those of irrigation specimens. In 
      several cases, irrigation FCM disclosed tumor stemlines that were not identified 
      in biopsy specimens. Discrepancies of this kind seemed most likely due to 
      differences in sampling. Irrigation FCM seems to be a sensitive method for 
      assessing multiple-site bladder tumors, and may be a useful technique for 
      monitoring the course of conservatively managed bladder tumors.
FAU - Collste, L G
AU  - Collste LG
FAU - Devonec, M
AU  - Devonec M
FAU - Darzynkiewicz, Z
AU  - Darzynkiewicz Z
FAU - Traganos, F
AU  - Traganos F
FAU - Sharpless, T K
AU  - Sharpless TK
FAU - Whitmore, W F Jr
AU  - Whitmore WF Jr
FAU - Melamed, M R
AU  - Melamed MR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Biopsy
MH  - Carcinoma, Papillary/*diagnosis/pathology
MH  - Computers
MH  - DNA/analysis
MH  - Humans
MH  - Ploidies
MH  - RNA/analysis
MH  - Therapeutic Irrigation
MH  - Urinary Bladder Neoplasms/*diagnosis/pathology
EDAT- 1980/05/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1980/05/01 00:00
PHST- 1980/05/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1980/05/01 00:00 [entrez]
AID - 10.1002/1097-0142(19800501)45:9<2389::aid-cncr2820450925>3.0.co;2-y [doi]
PST - ppublish
SO  - Cancer. 1980 May 1;45(9):2389-94. doi: 
      10.1002/1097-0142(19800501)45:9<2389::aid-cncr2820450925>3.0.co;2-y.