PMID- 6159446
OWN - NLM
STAT- MEDLINE
DCOM- 19810126
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 152
IP  - 5
DP  - 1980 Nov 1
TI  - Antigen- and receptor-driven regulatory mechanisms. V. The failure of 
      idiotype-coupled spleen cells to induce unresponsiveness in animals lacking the 
      appropriate VH genes is caused by the lack of idiotype-matched targets.
PG  - 1226-35
AB  - A/J anti-p-azobenzenearsonate (ABA) antibodies bearing cross-reactive idiotypic 
      (CRI) determinants, when coupled to spleen cells and then injected intravenously 
      into naive animals, stimulate suppressor T cell (Ts) responses. Moreover, 
      previous studies have demonstrated that the ability of such idiotype-coupled 
      spleen cells to induce immune unresponsiveness to subsequent immunization with 
      ABA-coupled spleen cells is linked to Igh-1 genes. Thus, CRI bearing antibodies 
      from A/J mice, when conjugated to normal BALB/c spleen cells in vitro and then 
      injected intravenously to syngeneic BALB/c mice, failed to induce tolerance in 
      these animals. However, spleen cells taken from these animals transferred 
      significant degrees of suppression to Igh-1 congenic C.AL-20 but not to H-2 
      congenic, Igh-1 distinct B10.D2 mice. Therefore, the failure of CRI-coupled 
      spleen cells to induce suppressor cell- mediated unresponsiveness in animals 
      unable to express the appropriate VH genes (i.e. BALB/c and B10.D2) appears to be 
      caused by the lack of idiotype- matched targets. The notion that the ability to 
      express certain Vn genes in the recipient animal is a prerequisite for suppressor 
      cell function was further supported by the observation that suppressor cells 
      induced in C.AL-20 mice failed to transfer any degree of suppression to BALB/c 
      mice. The ability to transfer suppression from BALB/c mice to C.AL-20 mice is a T 
      cell- dependent phenomenon, since in vitro treatment with anti-Thy 1.2 antiserum 
      and complement completely abrogated suppressor cell function. Furthermore, these 
      suppressor T cells are antigen specific and can be enriched on idiotype-coated 
      petri dishes, indicating they possess anti-idiotypic receptors. Therefore, 
      appropriate anti-idiotype and idiotype interaction is essential for the 
      manifestation of suppressor T cell function in ABA-specific suppressor pathways.
FAU - Sy, M S
AU  - Sy MS
FAU - Dietz, M H
AU  - Dietz MH
FAU - Nisonoff, A
AU  - Nisonoff A
FAU - Germain, R N
AU  - Germain RN
FAU - Benacerraf, B
AU  - Benacerraf B
FAU - Greene, M I
AU  - Greene MI
LA  - eng
GR  - AI-12907/AI/NIAID NIH HHS/United States
GR  - AI-14732/AI/NIAID NIH HHS/United States
GR  - AI-16396-01/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Epitopes)
RN  - 0 (Immunoglobulin Idiotypes)
SB  - IM
MH  - Animals
MH  - Cross Reactions
MH  - Epitopes/*genetics
MH  - Female
MH  - *Genes, MHC Class II
MH  - Hypersensitivity, Delayed/immunology
MH  - Immunoglobulin Idiotypes/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Spleen/cytology
MH  - T-Lymphocytes/*immunology
MH  - T-Lymphocytes, Regulatory/immunology
PMC - PMC2185996
EDAT- 1980/11/01 00:00
MHDA- 1980/11/01 00:01
PMCR- 1981/05/01
CRDT- 1980/11/01 00:00
PHST- 1980/11/01 00:00 [pubmed]
PHST- 1980/11/01 00:01 [medline]
PHST- 1980/11/01 00:00 [entrez]
PHST- 1981/05/01 00:00 [pmc-release]
AID - 15251226 [pii]
AID - 10.1084/jem.152.5.1226 [doi]
PST - ppublish
SO  - J Exp Med. 1980 Nov 1;152(5):1226-35. doi: 10.1084/jem.152.5.1226.