PMID- 6163065
OWN - NLM
STAT- MEDLINE
DCOM- 19810521
LR  - 20131121
IS  - 0044-376X (Print)
IS  - 0044-376X (Linking)
VI  - 84
IP  - 1
DP  - 1981 Jan
TI  - Acridine orange--its use in the specific staining of DNA in mammalian tissue 
      sections.
PG  - 37-42
AB  - This paper reports on a new method for the use of acridine orange (AO) in an 
      aqueous solution at pH 4.5 for staining DNA of rat tissue sections from which RNA 
      has been extracted selectively with cold phosphoric acid. Not only this, AO can 
      also be used as dye-SO2 reagent, prepared with NHCl and potassium metabisulphite, 
      for staining DNA-aldehyde molecules of acid-hydrolysed tissue sections. AO 
      samples, manufactured by the National Aniline Division as well as by G. T. Gurr 
      have been used with equal success. Studies of stained sections under light 
      microscope reveal the presence of specifically stained yellowish-orange nuclei. 
      Those sections under fluorescent microscope with proper exciter and barrier 
      filters reveal nuclei of maroon colour. The in situ absorption spectra of nuclei 
      stained with AO-SO2 following acid-hydrolysis of tissue sections as well as those 
      of nuclei stained with an aqueous solution of the dye following extraction of RNA 
      have been presented herein. The mode of binding in the former case has been 
      considered to be due to binding of the teritary amino group of the dye molecules 
      with the DNA-aldehyde molecules and in the latter case to be due to electrostatic 
      binding between the positively charged dye molecules with negatively charged 
      phosphate groups of DNA. Implications of all these findings have been discussed.
FAU - Dutt, M K
AU  - Dutt MK
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Microsc Acta
JT  - Microscopica acta
JID - 1306037
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Animals
MH  - Cell Nucleus/*analysis
MH  - DNA/*analysis
MH  - Kidney/analysis
MH  - Liver/analysis
MH  - Male
MH  - Rats
MH  - Staining and Labeling/*methods
MH  - Testis/analysis
EDAT- 1981/01/01 00:00
MHDA- 1981/01/01 00:01
CRDT- 1981/01/01 00:00
PHST- 1981/01/01 00:00 [pubmed]
PHST- 1981/01/01 00:01 [medline]
PHST- 1981/01/01 00:00 [entrez]
PST - ppublish
SO  - Microsc Acta. 1981 Jan;84(1):37-42.