PMID- 6166622
OWN - NLM
STAT- MEDLINE
DCOM- 19810925
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 89
IP  - 3
DP  - 1981 Jun
TI  - Human beta 2-microglobulin is a substrate of tissue transglutaminase: 
      polymerization in solution and on the cell surface.
PG  - 706-10
AB  - Incubation of purified human beta 2-microglobulin (B2-m) with tissue 
      transglutaminase (Tgase) resulted in the formation of high molecular weight 
      polymers revealed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. 
      In the presence of 30 mM [14C]methylamine, the polymer formation was prevented, 
      but incorporation of methylamine into beta 2-m (equal to 1 methylamine per 1 
      molecule) could be observed. From the sheddings of peripheral blood mononuclear 
      cells occurring in the presence of Tgase, it is apparent that anti-beta 2-m 
      immunoadsorbent removed, in addition to human leukocyte antigen (HLA) and beta 
      2-m, some other proteins. The enzyme could incorporate [14C]methylamine into beta 
      2-m of the shedding cells. On addition of rabbit anti-human beta 2-m antibody, 
      followed by fluoresceine-labeled goat anti-rabbit IgG antibody to human 
      mononuclear blood cells, the otherwise homogeneous distribution of fluorescence 
      turned into spots and patches on cells previously incubated with Tgase or 
      Ca2+-ionophore A23187.
FAU - Fesus, L
AU  - Fesus L
FAU - Falus, A
AU  - Falus A
FAU - Erdei, A
AU  - Erdei A
FAU - Laki, K
AU  - Laki K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Beta-Globulins)
RN  - 0 (Methylamines)
RN  - 0 (Polymers)
RN  - 0 (beta 2-Microglobulin)
RN  - EC 2.3.2.2 (gamma-Glutamyltransferase)
SB  - IM
MH  - Beta-Globulins/*metabolism
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Lymphocytes/*metabolism
MH  - Methylamines/metabolism
MH  - Polymers
MH  - beta 2-Microglobulin/immunology/*metabolism
MH  - gamma-Glutamyltransferase/*metabolism
PMC - PMC2111807
EDAT- 1981/06/01 00:00
MHDA- 1981/06/01 00:01
PMCR- 1981/12/01
CRDT- 1981/06/01 00:00
PHST- 1981/06/01 00:00 [pubmed]
PHST- 1981/06/01 00:01 [medline]
PHST- 1981/06/01 00:00 [entrez]
PHST- 1981/12/01 00:00 [pmc-release]
AID - 81239781 [pii]
AID - 10.1083/jcb.89.3.706 [doi]
PST - ppublish
SO  - J Cell Biol. 1981 Jun;89(3):706-10. doi: 10.1083/jcb.89.3.706.