PMID- 6166667
OWN - NLM
STAT- MEDLINE
DCOM- 19810915
LR  - 20190606
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 29
IP  - 6
DP  - 1981 Jun
TI  - Ultrastructural localization of complex carbohydrates in odontoblasts, predentin, 
      and dentin.
PG  - 747-58
AB  - Glycosaminoglycans (GAGs) and glycoproteins (GPs) are essential components for 
      dentinogenesis. We have examined rat odontoblasts, predentin, and dentin 
      decalcified with EDTA and stained with: 1) Spicer's hig-iron 
      diamine-thiocarbohydrazide-silver proteinate (HID-TCH-SP) method for sulfated 
      glycoconjugates, and 2) Thiery's periodate-thiocarbohydrazide-silver proteinate 
      (PA-TCH-SP) method for vicinal glycol-containing glycoconjugates. HIS-TCH-SP 
      stained distended portions of Golgi saccules and secretory granules. The 
      predentin contained three times the number of HID-TCH-SP stain precipitates when 
      compared to the mineralization front of the dentin matrix. PA-TCH-SP weakly 
      stained membranes of Golgi saccules and cisternae of rough endoplasmic reticulum 
      (RER), whereas stronger staining was observed in secretory granules, lysosomes, 
      and multivesicular bodies (MVBs). Collagen fibrils in predentin demonstrated 
      moderate PA-TCH-SP staining. In contrast, strong PA-TCH-SP staining was observed 
      on and between collagen fibrils in the mineralization front of the dentin matrix. 
      TCH-SP controls of unosmicated specimens lacked significant staining, however, 
      osmicated control specimens did contain some TCH-SP stain deposits in the 
      mineralization front. These results indicate that sulfated and vicinal 
      glycol-containing glycoconjugates are packaged in the same type of secretory 
      granule and released into the extracellular matrix; subsequently vicinal 
      glycol-containing glycoconjugates concentrate in the calcification front, whereas 
      sulfated glycoconjugates accumulate in the predentin and are either removed or 
      masked to staining in the dentin.
FAU - Takagi, M
AU  - Takagi M
FAU - Parmley, R T
AU  - Parmley RT
FAU - Denys, F R
AU  - Denys FR
LA  - eng
GR  - DE-02670/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (Glycoproteins)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Sulfates)
SB  - IM
MH  - Animals
MH  - Cytoplasmic Granules/analysis
MH  - Dentin/*analysis/ultrastructure
MH  - Endoplasmic Reticulum/analysis
MH  - Glycoproteins/*analysis
MH  - Glycosaminoglycans/*analysis
MH  - Golgi Apparatus/analysis
MH  - Histocytochemistry
MH  - Odontoblasts/*analysis/ultrastructure
MH  - Rats
MH  - Staining and Labeling
MH  - Sulfates/analysis
EDAT- 1981/06/01 00:00
MHDA- 1981/06/01 00:01
CRDT- 1981/06/01 00:00
PHST- 1981/06/01 00:00 [pubmed]
PHST- 1981/06/01 00:01 [medline]
PHST- 1981/06/01 00:00 [entrez]
AID - 10.1177/29.6.6166667 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 1981 Jun;29(6):747-58. doi: 10.1177/29.6.6166667.