PMID- 6170938
OWN - NLM
STAT- MEDLINE
DCOM- 19820120
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 9
IP  - 18
DP  - 1981 Sep 25
TI  - Purification and characterization of the messenger RNA for the heavy chain of rat 
      immunoglobulin E.
PG  - 4547-55
AB  - The isolation and translational properties of rat immunoglobulin E (IgE) heavy 
      chain mRNA are described. The mRNA has a sedimentation coefficient of 
      approximately 18S, a chain length of about 2000 nucleotides and directs the 
      synthesis in vitro of a polypeptide of 65000 molecular weight in an 
      mRNA-dependent rabbit reticulocyte lysate. Inclusion of dog pancreatic microsomes 
      in the cell-free translation system resulted in a heavy chain product of about 
      75000 molecular weight, presumably as a consequence of glycosylation in vitro. 
      This species co-migrated in an SDS polyacrylamide gel with mature IgE heavy 
      chain. Substantial purification of heavy chain mRNA was achieved by denaturing 
      sucrose gradient centrifugation and agarose gel electrophoresis.
FAU - Zajdel-Blair, M E
AU  - Zajdel-Blair ME
FAU - Blair, G E
AU  - Blair GE
FAU - Bennich, H
AU  - Bennich H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 0 (RNA, Messenger)
RN  - 24937-83-5 (Poly A)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Immunoglobulin E/*genetics
MH  - Immunoglobulin Heavy Chains/*genetics
MH  - Mice
MH  - Molecular Weight
MH  - Neoplasms, Experimental/immunology
MH  - Plasmacytoma/*immunology
MH  - Poly A/genetics
MH  - Protein Biosynthesis
MH  - RNA/genetics
MH  - RNA, Messenger/genetics/*isolation & purification
PMC - PMC327457
EDAT- 1981/09/25 00:00
MHDA- 1981/09/25 00:01
PMCR- 1981/09/25
CRDT- 1981/09/25 00:00
PHST- 1981/09/25 00:00 [pubmed]
PHST- 1981/09/25 00:01 [medline]
PHST- 1981/09/25 00:00 [entrez]
PHST- 1981/09/25 00:00 [pmc-release]
AID - 10.1093/nar/9.18.4547 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1981 Sep 25;9(18):4547-55. doi: 10.1093/nar/9.18.4547.