PMID- 6172558
OWN - NLM
STAT- MEDLINE
DCOM- 19820322
LR  - 20061115
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 57
IP  - Pt 2
DP  - 1981 Dec
TI  - Antigenic characterization of measles and SSPE virus haemagglutinin by monoclonal 
      antibodies.
PG  - 357-64
AB  - Hybrid cells secreting monoclonal antibodies directed against the haemagglutinin 
      (H) protein of measles virus (Edmonston) were produced by fusion of mouse myeloma 
      cells with spleen cells derived from immunized mice. Measles antibodies secreted 
      by these cells were tested for their ability to react with measles virus in 
      immunoprecipitation experiments and assays of binding, neutralization, 
      haemagglutination inhibition and haemolysin inhibition. On this basis 21 out of 
      75 hybridomas could be defined and divided into five functional groups with 
      different properties. However, when tested against other measles virus strains, 
      including those isolated from subacute sclerosing panencephalitis (SSPE) 
      patients, normalized radioimmunoassay (RIA) binding titres showed that the extent 
      to which a given antibody bound could vary greatly with the virus strain 
      examined. Moreover, the biological actions within a group were found to be very 
      heterogeneous, even when high antibody binding titres were observed. These 
      results suggest that different measles virus strains, which are not 
      distinguishable by polyvalent sera, do in fact possess antigenic differences. 
      Furthermore, the functional significance of a given virus epitope may vary from 
      strain to strain. Hybridoma antibodies were also used to demonstrate the 
      occurrence of antigenic changes within the H polypeptide of SSPE virus during the 
      course of non-productive, persistent infection in vitro.
FAU - ter Meulen, V
AU  - ter Meulen V
FAU - Loffler, S
AU  - Loffler S
FAU - Carter, M J
AU  - Carter MJ
FAU - Stephenson, J R
AU  - Stephenson JR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Epitopes)
RN  - 0 (Hemagglutinins, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Antibodies, Viral
MH  - Epitopes
MH  - Hemagglutination Inhibition Tests
MH  - Hemagglutinins, Viral/*immunology
MH  - Hybridomas
MH  - Measles virus/*immunology
MH  - Neutralization Tests
MH  - SSPE Virus/*immunology
MH  - Viral Proteins/immunology
EDAT- 1981/12/01 00:00
MHDA- 1981/12/01 00:01
CRDT- 1981/12/01 00:00
PHST- 1981/12/01 00:00 [pubmed]
PHST- 1981/12/01 00:01 [medline]
PHST- 1981/12/01 00:00 [entrez]
AID - 10.1099/0022-1317-57-2-357 [doi]
PST - ppublish
SO  - J Gen Virol. 1981 Dec;57(Pt 2):357-64. doi: 10.1099/0022-1317-57-2-357.