PMID- 6173179
OWN - NLM
STAT- MEDLINE
DCOM- 19820412
LR  - 20131121
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 2
IP  - 4
DP  - 1982 Jan
TI  - Luminescence of the solid complexes of acridine orange with RNA.
PG  - 201-11
AB  - The products of interaction between acridine orange (AO) and natural RNA, or the 
      synthetic RNA homopolymers are precipitates insoluble over a wide range of ionic 
      strength. These complexes have a composition of 1 AO molecule per 1 phosphate. 
      The reaction is highly cooperative and the complex exhibits metachromatic 
      luminescence. Significant differences in the luminescence spectra, related to 
      base composition of RNA, characterize the insoluble complexes. This observation 
      suggests that dye-base interactions take place in the AO-RNA complexes. During 
      titration of poly(rA) with AO and simultaneous measurement of the luminescence 
      and light scatter a two-step formation of the particles could be detected. The 
      cooperative binding of the ligand at D/P less than 0.6 coincides with formation 
      of small particles (molecular aggregates or micelles) and is followed at D/P 
      greater than 0.6 by a cooperative agglomeration; the product of this 
      agglomeration is particles of the size 0.35 micrometer and larger. Evidence is 
      presented that suggests that the long wave length luminescence (approximately 650 
      nm) is a consequence of the solute-solid state transition of the AO-RNA 
      interaction product rather than the classic dye-dye interactions previously 
      visualized in the stacking model. Since these novel observations cannot be fully 
      explained by the previously postulated molecular mechanisms of AO binding, an 
      alternative model is advanced. Its implications in quantitative cytochemistry of 
      nucleic acid as applied to flow cytometry are discussed.
FAU - Kapuscinski, J
AU  - Kapuscinski J
FAU - Darzynkiewicz, Z
AU  - Darzynkiewicz Z
FAU - Melamed, M R
AU  - Melamed MR
LA  - eng
GR  - 1-26-CA-14134/CA/NCI NIH HHS/United States
GR  - 1-R01-CA-23296/CA/NCI NIH HHS/United States
GR  - CA-28704/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (RNA, Ribosomal)
RN  - 24937-83-5 (Poly A)
RN  - 63231-63-0 (RNA)
RN  - 9014-25-9 (RNA, Transfer)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Animals
MH  - Chemical Phenomena
MH  - Chemistry
MH  - Cricetinae
MH  - Luminescent Measurements
MH  - Models, Chemical
MH  - Poly A
MH  - *RNA
MH  - *RNA, Ribosomal
MH  - *RNA, Transfer
MH  - Spectrophotometry
EDAT- 1982/01/01 00:00
MHDA- 1982/01/01 00:01
CRDT- 1982/01/01 00:00
PHST- 1982/01/01 00:00 [pubmed]
PHST- 1982/01/01 00:01 [medline]
PHST- 1982/01/01 00:00 [entrez]
AID - 10.1002/cyto.990020402 [doi]
PST - ppublish
SO  - Cytometry. 1982 Jan;2(4):201-11. doi: 10.1002/cyto.990020402.