PMID- 6175656
OWN - NLM
STAT- MEDLINE
DCOM- 19820624
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 15
IP  - 4
DP  - 1982 Apr
TI  - Comparison of acridine orange, methylene blue, and Gram stains for blood 
      cultures.
PG  - 562-6
AB  - Direct microscopic screening of blood cultures by Gram stain or methylene blue 
      stain is time consuming and frequently insensitive. Therefore, we evaluated a 
      fluorescent-staining procedure that uses acridine orange (AO) at pH 3.5 and 
      compared it with the methylene blue and Gram stain procedures. All smears were 
      prepared within 24 h of receiving the culture, fixed with methanol, and examined 
      without the results of the companion smears being known. AO-stained smears were 
      examined with incident-light fluorescence at 600 x magnification and confirmed at 
      1,500x magnification. All bottles macroscopically positive within 24 h were 
      excluded from the study. Of 2,946 cultures entered into the study, 204 (6.9%) 
      were positive within 3 days. The sensitivity and specificity of AO based on these 
      culture results were 52 and 98%, respectively, compared with 38% sensitivity and 
      99% specificity by methylene blue and Gram stains. The AO staining procedure is a 
      simple, sensitive, screening technique for the early detection of positive blood 
      cultures.
FAU - Mirrett, S
AU  - Mirrett S
FAU - Lauer, B A
AU  - Lauer BA
FAU - Miller, G A
AU  - Miller GA
FAU - Reller, L B
AU  - Reller LB
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - F30N4O6XVV (Acridine Orange)
RN  - T42P99266K (Methylene Blue)
SB  - IM
MH  - *Acridine Orange
MH  - Bacteria/*isolation & purification
MH  - Blood/*microbiology
MH  - False Negative Reactions
MH  - False Positive Reactions
MH  - Methylene Blue
MH  - Staining and Labeling/*methods
MH  - Yeasts/isolation & purification
PMC - PMC272144
EDAT- 1982/04/01 00:00
MHDA- 1982/04/01 00:01
PMCR- 1982/04/01
CRDT- 1982/04/01 00:00
PHST- 1982/04/01 00:00 [pubmed]
PHST- 1982/04/01 00:01 [medline]
PHST- 1982/04/01 00:00 [entrez]
PHST- 1982/04/01 00:00 [pmc-release]
AID - 10.1128/jcm.15.4.562-566.1982 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1982 Apr;15(4):562-6. doi: 10.1128/jcm.15.4.562-566.1982.