PMID- 6180875
OWN - NLM
STAT- MEDLINE
DCOM- 19821202
LR  - 20181130
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 3
IP  - 1
DP  - 1982 Jul
TI  - Permeabilization of lymphocytes with polyethylene glycol 1000. Discrimination of 
      permeabilized cells by flow cytometry.
PG  - 59-63
AB  - The toxicity of polyethylene glycol 1000 (PEG) used similarly as in cell 
      hybridization experiments, has been studied by flow cytometry, measuring the 
      light scattering and fluorescence distributions of PEG-treated human lymphocytes 
      stained with propidium iodide, fluorescein diacetate and acridine orange (AO). 
      The sensitivity of these tests to detect permeabilized, or potentially dead 
      cells, was equal. In addition, PEG proved to interfere with AO staining most 
      likely through the inhibition of its binding to nucleic acids. The decrease of AO 
      fluorescence in cells killed by PEG was unexpected since intercalation of 
      propidium iodide was the same as in alcohol fixed cells. Permeabilization of 
      cells by PEG appears to be an all-or-none phenomenon, accompanied by entrance of 
      PEG into the cells. The findings are described in the context of a review of the 
      currently used flow cytometric techniques to discriminate viable and lethally 
      affected cells; also, the problems of interpretation are discussed.
FAU - Szabo, G Jr
AU  - Szabo G Jr
FAU - Kiss, A
AU  - Kiss A
FAU - Tron, L
AU  - Tron L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Cell Membrane Permeability/*drug effects
MH  - Cell Survival
MH  - Flow Cytometry
MH  - Humans
MH  - Light
MH  - Lymphocytes/drug effects/*physiology
MH  - Polyethylene Glycols/*pharmacology
MH  - Scattering, Radiation
MH  - Staining and Labeling
EDAT- 1982/07/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1982/07/01 00:00
PHST- 1982/07/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1982/07/01 00:00 [entrez]
AID - 10.1002/cyto.990030113 [doi]
PST - ppublish
SO  - Cytometry. 1982 Jul;3(1):59-63. doi: 10.1002/cyto.990030113.