PMID- 6180922
OWN - NLM
STAT- MEDLINE
DCOM- 19821202
LR  - 20141120
IS  - 0301-472X (Print)
IS  - 0301-472X (Linking)
VI  - 10
IP  - 6
DP  - 1982 Jul
TI  - Studies on the exocytosis of cultured mast cells derived from mouse bone marrow.
PG  - 524-32
AB  - Mast cells were obtained from mouse bone marrow cells cultured for 14 days in 
      medium derived from Concanavalin A (Con A) stimulated mouse spleen cells. Upon 
      passive sensitization of the cultured cells with immunoglobulin E (IgE), 
      histamine release from mast cells was approximately 200% above control within 1 
      min of incubation with anti-IgE. The calcium inonophore A 23187 also evoked a 
      concentration-dependent (10(-8) M to 6 x 10(-7) M) histamine release following a 
      6 min incubation. Transmission electron microscopy (TEM) demonstrated that the 
      secretory granules of the cultured cells have a peripheral crystalline pattern, 
      like that previously demonstrated for mast cells. Scanning electron microscopy 
      (SEM) illustrated spherical cells with surfaces traversed by many ridge-like 
      folds. Intragranular fusion, following exposure of the IgE-bearing cells to 
      anti-IgE, led to accumulation of the granules into channels which, on study with 
      both transmission and scanning electron microscopy, appeared to be associated 
      with the cell surface.
FAU - Razin, E
AU  - Razin E
FAU - Cordon-Cardo, A
AU  - Cordon-Cardo A
FAU - Minick, C R
AU  - Minick CR
FAU - Good, R A
AU  - Good RA
LA  - eng
GR  - AI-11843/AI/NIAID NIH HHS/United States
GR  - HL-18828/HL/NHLBI NIH HHS/United States
GR  - NS-11457/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Exp Hematol
JT  - Experimental hematology
JID - 0402313
RN  - 0 (Culture Media)
RN  - 11028-71-0 (Concanavalin A)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 37H9VM9WZL (Calcimycin)
SB  - IM
MH  - Animals
MH  - *Bone Marrow Cells
MH  - Calcimycin/pharmacology
MH  - Cells, Cultured
MH  - Concanavalin A/pharmacology
MH  - Culture Media
MH  - Cytoplasmic Granules/ultrastructure
MH  - Exocytosis
MH  - *Histamine Release/drug effects
MH  - Immunoglobulin E/immunology
MH  - In Vitro Techniques
MH  - Male
MH  - Mast Cells/*physiology/ultrastructure
MH  - Mice
MH  - Microscopy, Electron, Scanning
EDAT- 1982/07/01 00:00
MHDA- 1982/07/01 00:01
CRDT- 1982/07/01 00:00
PHST- 1982/07/01 00:00 [pubmed]
PHST- 1982/07/01 00:01 [medline]
PHST- 1982/07/01 00:00 [entrez]
PST - ppublish
SO  - Exp Hematol. 1982 Jul;10(6):524-32.