PMID- 6184042
OWN - NLM
STAT- MEDLINE
DCOM- 19830119
LR  - 20190904
IS  - 0004-945X (Print)
IS  - 0004-945X (Linking)
VI  - 60 Pt 4
DP  - 1982 Aug
TI  - A monoclonal anti-idiotope antibody reacting with a determinant of the 
      cross-reactive idiotype on anti-p-azobenzenearsonate antibodies of A/J mice.
PG  - 369-82
AB  - A hybrid cell line, 14A1, was produced that secretes antibodies reacting with a 
      monoclonal A/J anti-p-azobenzenearsonate (ABA) antibody, 7.1.3, previously shown 
      to have all of the determinants of the cross-reactive idiotype (CRI) defined by 
      rabbit anti-idiotype sera. 14A1 were detected using a sensitive assay that is 
      specific for antibodies against idiotypic, but not allotypic nor isotypic, 
      determinants. This was confirmed by demonstrating that 14A1 antibodies bind 7.1.3 
      but not other A/J monoclonal antibodies of the same class or CRI- monoclonal 
      anti-ABA antibodies. The idiotypic determinant recognized by 14A1 is located at 
      or near the hapten-binding site of 7.1.3, as ABA-conjugated tyrosine is capable 
      of blocking this interaction. RAbbit anti-CRI antibodies are prevented from 
      binding to 7.1.3 by the addition of 14A1, demonstrating the spatial proximity of 
      the 14A1 idiotope with determinant(s) of the CRI. All A/J anti-ABA antibodies 
      that have the 14A1 idiotope are also CRI+, while anti-ABA antibodies from other 
      strains of mice to not express this idiotope. Furthermore, antibodies from A/J 
      mice immunologically suppressed for the CRI also fail to interact with 14A1 
      antibodies. These results have implications both for the nature of the CRI and 
      for the regulation of the immune response by anti-idiotype antibodies.
FAU - Morahan, G
AU  - Morahan G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Aust J Exp Biol Med Sci
JT  - The Australian journal of experimental biology and medical science
JID - 0416662
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Azo Compounds)
RN  - 0 (Epitopes)
RN  - 0 (Immunoglobulin Idiotypes)
RN  - 7334-23-8 (p-Azobenzenearsonate)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Antibody Specificity
MH  - Azo Compounds/*immunology
MH  - Binding, Competitive
MH  - Cross Reactions
MH  - Epitopes
MH  - Hybridomas/immunology
MH  - Immunoglobulin Idiotypes/*immunology
MH  - Mice
MH  - Mice, Inbred A
MH  - p-Azobenzenearsonate/*immunology
EDAT- 1982/08/01 00:00
MHDA- 1982/08/01 00:01
CRDT- 1982/08/01 00:00
PHST- 1982/08/01 00:00 [pubmed]
PHST- 1982/08/01 00:01 [medline]
PHST- 1982/08/01 00:00 [entrez]
AID - 10.1038/icb.1982.41 [doi]
PST - ppublish
SO  - Aust J Exp Biol Med Sci. 1982 Aug;60 Pt 4:369-82. doi: 10.1038/icb.1982.41.