PMID- 6186729
OWN - NLM
STAT- MEDLINE
DCOM- 19830407
LR  - 20071114
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 31
IP  - 1A Suppl
DP  - 1983 Jan
TI  - Rapid analysis of normal and abnormal cell types in human semen and testis 
      biopsies by flow cytometry.
PG  - 248-53
AB  - A simple, rapid procedure is described that quantitates RNA content and DNA 
      content/chromatin condensation for each of many possible cell types and 
      differentiation levels of the cells present in human semen. A fresh semen sample 
      (1-6 hr postemission) or frozen sample (allowing samples to be accumulated and 
      sent to a laboratory) is treated with a detergent solution, stained with acridine 
      orange (AO), and measured by flow cytometry (FCM); approximately 10 minutes are 
      required to measure 5,000 cells per sample and analyze the data with computer 
      assistance. The following can be learned from a single measurement: a) the 
      percentage of each cell type in semen including, i) mature sperm, ii) immature 
      sperm precursor cells, representing all stages of development from spermatogonia 
      to mature sperm, iii) somatic cells, e.g., leukocytes; b) normality/abnormality 
      of sperm nuclear chromatin condensation. These measurements can be correlated 
      with cell types in testis biopsies identified by two-parameter FCM measurements 
      (RNA versus DNA) using AO as the fluorescent probe.
FAU - Evenson, D P
AU  - Evenson DP
FAU - Melamed, M R
AU  - Melamed MR
LA  - eng
GR  - CA-08748/CA/NCI NIH HHS/United States
GR  - HD12196/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (Chromatin)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Chromatin/ultrastructure
MH  - Flow Cytometry
MH  - Haploidy
MH  - Humans
MH  - Male
MH  - RNA/metabolism
MH  - Semen/*cytology
MH  - Staining and Labeling
MH  - Testis/*cytology/pathology
EDAT- 1983/01/01 00:00
MHDA- 1983/01/01 00:01
CRDT- 1983/01/01 00:00
PHST- 1983/01/01 00:00 [pubmed]
PHST- 1983/01/01 00:01 [medline]
PHST- 1983/01/01 00:00 [entrez]
PST - ppublish
SO  - J Histochem Cytochem. 1983 Jan;31(1A Suppl):248-53.