PMID- 6188072
OWN - NLM
STAT- MEDLINE
DCOM- 19830527
LR  - 20190726
IS  - 0028-3908 (Print)
IS  - 0028-3908 (Linking)
VI  - 22
IP  - 2
DP  - 1983 Feb
TI  - Structure-activity relationship in the mast cell degranulating capacity of 
      neurotensin fragments.
PG  - 197-201
AB  - The mast cell degranulating capacity of neurotensin and three of its fragments 
      was examined. In Tyrode solution (137 mM NaCl, 2.7 mM KCl, 0.4 mM NaH2PO4, 1.4 mM 
      CaCl2, 1 mM MgCl2, 10 mM Hepes, 5.6 mM glucose, pH 7.4), neither intact 
      neurotensin nor its C-terminal tripeptide (Tyr-Ile-Leu) caused any release of 
      histamine. Concentrations of neurotensin exceeding 10(-4)M did cause histamine 
      release but through lysis of the cells. The C-terminal hexa- and octapeptides of 
      neurotensin (Arg-Arg-Pro-Tyr-Ile-Leu and Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu, 
      respectively) induced a non-cytolytic release of histamine with the latter 
      peptide being more active (ED50 = 90 microM for the hexapeptide and 13 microM for 
      the octapeptide). This release was not affected by the C-terminal tripeptide. It 
      was found to be calcium-dependent and was inhibited by the anti-allergic drug, 
      disodium cromoglycate. Phosphatidylserine did not enhance release of histamine 
      and saturation of the immunoglobulin E (IgE) receptors did not inhibit it.
FAU - Sagi-Eisenberg, R
AU  - Sagi-Eisenberg R
FAU - Ben-Neriah, Z
AU  - Ben-Neriah Z
FAU - Pecht, I
AU  - Pecht I
FAU - Terry, S
AU  - Terry S
FAU - Blumberg, S
AU  - Blumberg S
LA  - eng
PT  - Journal Article
PL  - England
TA  - Neuropharmacology
JT  - Neuropharmacology
JID - 0236217
RN  - 0 (Peptide Fragments)
RN  - 0 (Phosphatidylserines)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 39379-15-2 (Neurotensin)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/physiology
MH  - Cytoplasmic Granules/*drug effects
MH  - Histamine Release/drug effects
MH  - Immunoglobulin E/pharmacology
MH  - In Vitro Techniques
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Male
MH  - Mast Cells/*ultrastructure
MH  - Neurotensin/*analogs & derivatives/pharmacology
MH  - Peptide Fragments/pharmacology
MH  - Phosphatidylserines/pharmacology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Structure-Activity Relationship
EDAT- 1983/02/01 00:00
MHDA- 1983/02/01 00:01
CRDT- 1983/02/01 00:00
PHST- 1983/02/01 00:00 [pubmed]
PHST- 1983/02/01 00:01 [medline]
PHST- 1983/02/01 00:00 [entrez]
AID - 10.1016/0028-3908(83)90009-6 [doi]
PST - ppublish
SO  - Neuropharmacology. 1983 Feb;22(2):197-201. doi: 10.1016/0028-3908(83)90009-6.