PMID- 6188762
OWN - NLM
STAT- MEDLINE
DCOM- 19830610
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 17
IP  - 3
DP  - 1983 Mar
TI  - Acridine orange staining and radiometric detection of microorganisms in blood 
      cultures.
PG  - 463-5
AB  - To determine whether acridine orange (AO) staining of blood cultures could be 
      used as a substitute for blind subculture when used in conjunction with the 
      BACTEC system (Johnston Laboratories, Inc., Towson, Md.), the two methods were 
      compared on all BACTEC-negative specimens. Since blind subcultures were routinely 
      performed in our laboratory on days 2 and 6 of incubation, AO staining was also 
      performed on these days. Cultures which were BACTEC positive on day 1 of 
      incubation were not included in the study. Of the 2,395 bottles tested after 2 
      days of incubation, 106 were subculture positive. Of these, 96 (90.6%) were also 
      AO positive and BACTEC positive, 3 (2.8%) were AO positive and BACTEC negative, 
      and 7 (6.6%) were AO negative and BACTEC positive. Of the 3,487 bottles tested on 
      day 6 of incubation, 14 were subculture positive; 7 (50%) of these were AO 
      positive and BACTEC positive, and seven were AO positive and BACTEC negative. Of 
      the total of 10 culture-positive bottles missed by BACTEC, all were positive, and 
      all 10 companion aerobic bottles were BACTEC positive. In both phases of the 
      experiment, there was a total of only four false-positive AO stains. As a result 
      of this investigation, we have substituted AO staining for blind subculturing of 
      BACTEC-negative bottles.
FAU - Burdash, N M
AU  - Burdash NM
FAU - Manos, J P
AU  - Manos JP
FAU - Bannister, E R
AU  - Bannister ER
FAU - Welborn, A L
AU  - Welborn AL
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Culture Media)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Blood/*microbiology
MH  - Culture Media
MH  - Humans
MH  - Radiometry
MH  - Staining and Labeling/*methods
PMC - PMC272666
EDAT- 1983/03/01 00:00
MHDA- 1983/03/01 00:01
PMCR- 1983/03/01
CRDT- 1983/03/01 00:00
PHST- 1983/03/01 00:00 [pubmed]
PHST- 1983/03/01 00:01 [medline]
PHST- 1983/03/01 00:00 [entrez]
PHST- 1983/03/01 00:00 [pmc-release]
AID - 10.1128/jcm.17.3.463-465.1983 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1983 Mar;17(3):463-5. doi: 10.1128/jcm.17.3.463-465.1983.