PMID- 6190168
OWN - NLM
STAT- MEDLINE
DCOM- 19830708
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 80
IP  - 10
DP  - 1983 May
TI  - Selective phosphorylation of the IgE receptor in antigen-stimulated rat mast 
      cells.
PG  - 3050-3
AB  - Purified rat serosal mast cells were sensitized with mouse immunoglobulin E (IgE) 
      anti-2,4-dinitrophenyl antibody, partially depleted of phosphate, labeled with 
      [32P]orthophosphate, and stimulated with dinitrophenylated bovine serum albumin 
      or control protein. After 15-120 seconds at 37 degrees C, the cells were 
      extracted with nonionic detergent. IgE receptors were purified by repetitive 
      affinity chromatography and were analyzed by NaDodSO4/polyacrylamide gel 
      electrophoresis and radioautography. Antigenic stimulation of intact rat mast 
      cells produced a rapid and marked increase in the phosphorylation of the 
      surface-exposed alpha component of the IgE receptor. However, phosphorylation of 
      the 33,000 Mr beta component of the IgE receptor was not altered significantly by 
      antigen stimulation. This suggests that the selective increase in phosphorylation 
      of the IgE receptor alpha component may be part of the physiologic mediator 
      secretion process triggered by antigen.
FAU - Hempstead, B L
AU  - Hempstead BL
FAU - Parker, C W
AU  - Parker CW
FAU - Kulczycki, A Jr
AU  - Kulczycki A Jr
LA  - eng
GR  - P50 AI 15322/AI/NIAID NIH HHS/United States
GR  - R01 AI 16946/AI/NIAID NIH HHS/United States
GR  - T32 GM 7200/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Antigens)
RN  - 0 (Glycoproteins)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Receptors, IgE)
RN  - 0 (Receptors, Immunologic)
SB  - IM
MH  - Animals
MH  - Antigens
MH  - Glycoproteins/metabolism
MH  - Histamine Release
MH  - Kinetics
MH  - Macromolecular Substances
MH  - Mast Cells/*immunology
MH  - Molecular Weight
MH  - Phosphorylation
MH  - Rats
MH  - Receptors, IgE
MH  - Receptors, Immunologic/*metabolism
PMC - PMC393971
EDAT- 1983/05/01 00:00
MHDA- 2001/03/28 10:01
PMCR- 1983/11/01
CRDT- 1983/05/01 00:00
PHST- 1983/05/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1983/05/01 00:00 [entrez]
PHST- 1983/11/01 00:00 [pmc-release]
AID - 10.1073/pnas.80.10.3050 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1983 May;80(10):3050-3. doi: 10.1073/pnas.80.10.3050.