PMID- 6190910
OWN - NLM
STAT- MEDLINE
DCOM- 19830811
LR  - 20171116
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 131
IP  - 1
DP  - 1983 Jul
TI  - A rabbit B cell determinant for a conserved portion of myelin basic protein, 
      rabbit encephalitogenic sequence 65-74.
PG  - 275-81
AB  - Synthetic peptide S24 (TTHYGSLPQKG) represents residues 65-74 of myelin basic 
      protein (MBP) and contains the major determinant involved in the development of 
      experimental allergic encephalomyelitis (EAE) in rabbits. This peptide is 
      completely conserved in all nonprimate mammals for which sequence information is 
      available. Although it is clear that peptides containing the S24 region are 
      capable of inducing EAE, previous serologic studies have resulted in the 
      conclusion that the determinant is "buried" or sequestered in intact MBP. 
      Employing a liquid phase radioimmunoassay, we studied Ab responses to the S24 
      determinant in six rabbits injected with rat myelin. Two of the six animals 
      developed small but measurable responses to the S24 determinant. In one of these 
      rabbits, the response was boosted with a covalent conjugate of S82 and methylated 
      BSA (MBSA). We also measured antibodies to the S24 determinant in rabbit antisera 
      to human, monkey, dog, bovine, and the large and small forms of rat MBP. By 
      nonequilibrium inhibition analysis, we determined that the antibody responses to 
      these antigens were all directed to a determinant composed of residues 66-71 of 
      MBP, and that intact MBP inhibits the binding of these antibodies to radiolabeled 
      S24. The results demonstrate that the rabbit encephalitogenic region of myelin 
      basic protein is exposed in the intact molecule both as an immunogen and as a 
      reactant in liquid-phase assays; furthermore, they demonstrate that MBP 
      antigenicity leading to B cell responses does not necessarily depend on sequence 
      differences between the injected protein and its counterpart in the host species. 
      The latter finding reinforces the contention of Atassi that autoantibody 
      responses are not exclusive to "evolutionary hypervariable locations."
FAU - Lazarus, K J
AU  - Lazarus KJ
FAU - Hashim, G A
AU  - Hashim GA
FAU - Varitek, V A Jr
AU  - Varitek VA Jr
FAU - Paterson, P Y
AU  - Paterson PY
FAU - Day, E D
AU  - Day ED
LA  - eng
GR  - NS-06262/NS/NINDS NIH HHS/United States
GR  - NS-10237/NS/NINDS NIH HHS/United States
GR  - NS-15322/NS/NINDS NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Epitopes)
RN  - 0 (Immune Sera)
RN  - 0 (Myelin Basic Protein)
RN  - 0 (methylated bovine serum albumin)
RN  - 27432CM55Q (Serum Albumin, Bovine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibody Formation
MH  - B-Lymphocytes/*immunology
MH  - Binding Sites, Antibody
MH  - Binding, Competitive
MH  - Cattle
MH  - Dogs
MH  - Epitopes/*immunology
MH  - Haplorhini
MH  - Humans
MH  - Immune Sera/analysis
MH  - Immunization, Secondary
MH  - Myelin Basic Protein/*immunology
MH  - Rabbits
MH  - Rats
MH  - Serum Albumin, Bovine/administration & dosage
EDAT- 1983/07/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1983/07/01 00:00
PHST- 1983/07/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1983/07/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1983 Jul;131(1):275-81.