PMID- 6192893
OWN - NLM
STAT- MEDLINE
DCOM- 19831028
LR  - 20190720
IS  - 0008-4166 (Print)
IS  - 0008-4166 (Linking)
VI  - 29
IP  - 6
DP  - 1983 Jun
TI  - The detection of positive blood cultures in children using the acridine orange, 
      gram, and methylene blue stains.
PG  - 729-31
AB  - The efficacy of the acridine orange (AO), gram (G), and methylene blue (MB) 
      microscopic procedures was analyzed using positive blood cultures monitored by a 
      radiometric procedure (Bactec) in children. Sixty-eight positive cultures 
      included the following isolates: Haemophilus influenzae type b, Neisseria 
      meningitidis, Streptococcus pneumoniae, Enterobacteriaceae, Staphylococcus 
      aureus, Candida spp., and seven other pathogens. The MB stain yielded the highest 
      detection rate, 99%, in comparison with 94 and 93% for the AO and G stains, 
      respectively. Since the MB stain yielded comparable results to the AO procedure 
      with no requirement for a fluorescent microscope, the former method is 
      recommended for confirming the presence and initial characterization of 
      microorganisms in blood cultures monitored by Bactec from children.
FAU - La Scolea, L J Jr
AU  - La Scolea LJ Jr
FAU - Baldigo, S M
AU  - Baldigo SM
LA  - eng
PT  - Journal Article
PL  - Canada
TA  - Can J Microbiol
JT  - Canadian journal of microbiology
JID - 0372707
RN  - F30N4O6XVV (Acridine Orange)
RN  - T42P99266K (Methylene Blue)
SB  - IM
MH  - *Acridine Orange
MH  - Adolescent
MH  - Bacteria/*isolation & purification
MH  - Blood/*microbiology
MH  - Child
MH  - Child, Preschool
MH  - Humans
MH  - Infant
MH  - *Methylene Blue
MH  - Sepsis/*microbiology
MH  - Staining and Labeling
EDAT- 1983/06/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1983/06/01 00:00
PHST- 1983/06/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1983/06/01 00:00 [entrez]
AID - 10.1139/m83-119 [doi]
PST - ppublish
SO  - Can J Microbiol. 1983 Jun;29(6):729-31. doi: 10.1139/m83-119.