PMID- 6193130
OWN - NLM
STAT- MEDLINE
DCOM- 19831028
LR  - 20151119
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 116
IP  - 3
DP  - 1983 Sep
TI  - Mitogenic effect of prostaglandin E1 in Swiss 3T3 cells: role of cyclic AMP.
PG  - 379-84
AB  - Addition of prostaglandin E1 (PGE1) to quiescent cultures of Swiss 3T3 cells 
      rapidly elevates the intracellular levels of cAMP and increases the activity of 
      adenylate cyclase in particulate fractions of these cells. In the presence of 
      insulin, PGE1 stimulates the reinitiation of DNA synthesis. Both effects 
      (increase in cellular cAMP and stimulation of DNA synthesis) are markedly 
      potentiated by 1-methyl-3-isobutyl xanthine (IBMX) or by 4-(3-butoxy-4 methoxy 
      benzyl)-2-imidazolidine (Ro 20-1724), both of which are potent inhibitors of 
      cyclic nucleotide phosphodiesterase activity. In the presence of 50 microM IBMX, 
      PGE1 caused a dose-dependent increase in cAMP levels and in [3H]thymidine 
      incorporation into acid-insoluble material at concentrations (5-50 ng/ml) that 
      are orders of magnitude lower than those used in previous studies (50 
      micrograms/ml) to demonstrate growth-inhibitory effects. Thus, the inhibitory 
      effects produced by adding high concentrations of PGE1 on the initiation of DNA 
      synthesis in Swiss 3T3 cells are not mediated by cAMP and should be regarded as 
      nonspecific. In contrast, the mitogenic activity of PGE1 parallels its ability to 
      increase the intracellular levels of cAMP. The findings support the proposition 
      that a sustained increase in the level of this cyclic nucleotide acts as a 
      mitogenic signal for confluent and quiescent Swiss 3T3 cells.
FAU - Rozengurt, E
AU  - Rozengurt E
FAU - Collins, M K
AU  - Collins MK
FAU - Keehan, M
AU  - Keehan M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Insulin)
RN  - 0 (Mitogens)
RN  - 0 (Prostaglandins E)
RN  - 9007-49-2 (DNA)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
RN  - F5TD010360 (Alprostadil)
RN  - K7Q1JQR04M (Dinoprostone)
RN  - TBT296U68M (1-Methyl-3-isobutylxanthine)
SB  - IM
MH  - 1-Methyl-3-isobutylxanthine/pharmacology
MH  - Adenylyl Cyclases/metabolism
MH  - Alprostadil
MH  - Animals
MH  - Cell Line
MH  - Cells, Cultured
MH  - Cyclic AMP/*metabolism
MH  - DNA/biosynthesis
MH  - Dinoprostone
MH  - Dose-Response Relationship, Drug
MH  - Drug Interactions
MH  - Insulin/pharmacology
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Mitogens/*pharmacology
MH  - Prostaglandins E/*pharmacology
EDAT- 1983/09/01 00:00
MHDA- 1983/09/01 00:01
CRDT- 1983/09/01 00:00
PHST- 1983/09/01 00:00 [pubmed]
PHST- 1983/09/01 00:01 [medline]
PHST- 1983/09/01 00:00 [entrez]
AID - 10.1002/jcp.1041160316 [doi]
PST - ppublish
SO  - J Cell Physiol. 1983 Sep;116(3):379-84. doi: 10.1002/jcp.1041160316.