PMID- 6193174
OWN - NLM
STAT- MEDLINE
DCOM- 19831008
LR  - 20171116
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 131
IP  - 3
DP  - 1983 Sep
TI  - Contact hypersensitivity reactions to dinitrofluorobenzene mediated by monoclonal 
      IgE anti-DNP antibodies.
PG  - 1096-102
AB  - Several studies have suggested a possible role for IgE antibodies in the 
      pathogenesis of cutaneous hypersensitivity reactions that reach maximum intensity 
      24 to 48 hr after antigen challenge. The recent availability of murine monoclonal 
      IgE anti-hapten antibodies has made possible the direct examination of the range 
      of cutaneous inflammatory reactions that can be mediated by such antibodies. We 
      have examined the effects of passively sensitizing BALB/c mice with monoclonal 
      IgE anti-dinitrophenyl (DNP) antibody 48 hr before antigen challenge. 
      Inflammatory responses were assessed by measuring ear swelling in mice challenged 
      on the ears with the reactive hapten 2,4-dinitrofluorobenzene (DNFB). Compared 
      with unsensitized controls, the ears of mice passively sensitized with IgE 
      anti-DNP displayed a biphasic pattern of ear swelling after DNFB challenge. An 
      early, transient response (present within 15 to 30 min of challenge and returning 
      to control levels within 4 to 9 hr) was followed by a second, more persistent 
      increase in ear swelling that peaked 24 to 48 hr after challenge. This biphasic 
      pattern of ear swelling seen in IgE-sensitized mice was temporally 
      indistinguishable from that observed in mice conventionally sensitized for 
      allergic contact dermatitis reactions by epicutaneous application of DNFB 5 days 
      before DNFB ear challenge. Antigen specificity of the IgE-mediated contact 
      hypersensitivity reactions was demonstrated by the failure of mice passively 
      sensitized with IgE anti-DNP to display early or delayed ear swelling greater 
      than unsensitized controls when challenged with either of two noncross-reacting 
      haptens, fluorescein isothiocyanate or oxazolone. Mice passively sensitized with 
      a monoclonal IgA anti-DNP antibody (MOPC 315) 48 hr before DNFB challenge failed 
      to display early or delayed ear swelling greater than unsensitized controls. Heat 
      inactivation of the IgE anti-DNP ascitic fluid at 56 degrees C for 30 min 
      completely abolished its capacity to passively sensitize mice for contact 
      hypersensitivity reactions after DNFB challenge. These results document the 
      existence of an antigen-specific, IgE-mediated, delayed-in-time cutaneous 
      hypersensitivity response that can be elicited by epicutaneous challenge 
      (contract) with a reactive hapten.
FAU - Ray, M C
AU  - Ray MC
FAU - Tharp, M D
AU  - Tharp MD
FAU - Sullivan, T J
AU  - Sullivan TJ
FAU - Tigelaar, R E
AU  - Tigelaar RE
LA  - eng
GR  - AI 17363/AI/NIAID NIH HHS/United States
GR  - AM 07341/AM/NIADDK NIH HHS/United States
GR  - AM 28425/AM/NIADDK NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Benzenesulfonates)
RN  - 0 (Epitopes)
RN  - 0 (Nitrobenzenes)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 3WF0U675G6 (2,4-dinitrobenzenesulfonic acid)
RN  - D241E059U6 (Dinitrofluorobenzene)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*administration & dosage/physiology
MH  - Benzenesulfonates/*immunology
MH  - Dermatitis, Contact/diagnosis/*immunology
MH  - Dinitrofluorobenzene/*administration & dosage
MH  - Epitopes
MH  - Female
MH  - Hot Temperature
MH  - Immunization, Passive
MH  - Immunoglobulin E/administration & dosage
MH  - Kinetics
MH  - Lymph Nodes/cytology/immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nitrobenzenes/*administration & dosage
MH  - Rats
MH  - Rats, Inbred Strains
EDAT- 1983/09/01 00:00
MHDA- 1983/09/01 00:01
CRDT- 1983/09/01 00:00
PHST- 1983/09/01 00:00 [pubmed]
PHST- 1983/09/01 00:01 [medline]
PHST- 1983/09/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1983 Sep;131(3):1096-102.