PMID- 6194224
OWN - NLM
STAT- MEDLINE
DCOM- 19831123
LR  - 20071115
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 131
IP  - 4
DP  - 1983 Oct
TI  - Characterization of alloimmunization-induced T lymphocytes reactive against AKR 
      leukemia in vitro and correlation with graft-vs-leukemia activity in vivo.
PG  - 2050-8
AB  - We have reported that immunization of H-2k mice with lymphoid cells from various 
      allogeneic strains induced a population of cells that could eliminate 
      first-passage spontaneous AKR leukemia from the spleens of immuno-suppressed AKR 
      (H-2k) hosts. In the present study, we examined the nature of the cells 
      responsible for this graft-vs-leukemia (GVL) reaction and compared them to 
      cytolytic cells detected in vitro. Spleen cells from alloimmunized CBA/J (H-2k) 
      mice were selectively depleted of various subpopulations by treatment with 
      antibody and complement (C), then tested in vivo for GVL reactivity. Cell 
      suspensions depleted of Thy-1.2+, Lyt-1+, or Lyt-2+ lymphocytes had no 
      significant GVL reactivity, whereas suspensions depleted of NK-1.2+ cells 
      retained GVL reactivity. The GVL-reactive cells persisted in H-2-compatible donor 
      mice for up to 56 days. Lyt-1+2+ lymphocytes that were cytotoxic for cultured AKR 
      leukemia cells in vitro could be detected in the spleens of alloimmunized 
      H-2-compatible mice after expansion of the cells in T cell growth factor. Using 
      quantitative limiting dilution cytotoxicity assays, we found that the frequency 
      of leukemia-reactive cytotoxic lymphocytes (CL) in the spleen showed a direct 
      correlation with the GVL efficacy of the cells in vivo. Alloimmunization was 
      essential for induction of the GVL-reactive cell population. CL in alloimmunized 
      mice consisted of heterogeneous cytotoxic specificities; i.e., some CL were 
      leukemia-specific, others lysed only nonleukemic AKR target cells, and a third 
      group mediated killing of both leukemic and nonleukemic target cells. The CL 
      appeared to be H-2 restricted and specific for non-H-2 antigens shared by the AKR 
      leukemia and the alloimmunizing cells.
FAU - Truitt, R L
AU  - Truitt RL
FAU - Shih, C Y
AU  - Shih CY
FAU - Lefever, A V
AU  - Lefever AV
FAU - Tempelis, L D
AU  - Tempelis LD
FAU - Andreani, M
AU  - Andreani M
FAU - Bortin, M M
AU  - Bortin MM
LA  - eng
GR  - CA 20484/CA/NCI NIH HHS/United States
GR  - CA 26245/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Epitopes)
RN  - 0 (H-2 Antigens)
SB  - IM
MH  - Animals
MH  - Cytotoxicity, Immunologic
MH  - Dose-Response Relationship, Immunologic
MH  - Epitopes/analysis
MH  - *Graft vs Host Reaction
MH  - H-2 Antigens/administration & dosage
MH  - Immunologic Memory
MH  - Leukemia, Experimental/*immunology
MH  - Leukemia, Lymphoid/*immunology
MH  - Lymphocyte Depletion
MH  - Lymphocyte Transfusion
MH  - Lymphocytes/immunology
MH  - Mice
MH  - Mice, Inbred AKR
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred CBA
MH  - Mice, Inbred DBA
MH  - T-Lymphocytes/classification/*immunology
MH  - T-Lymphocytes, Cytotoxic/immunology/transplantation
EDAT- 1983/10/01 00:00
MHDA- 1983/10/01 00:01
CRDT- 1983/10/01 00:00
PHST- 1983/10/01 00:00 [pubmed]
PHST- 1983/10/01 00:01 [medline]
PHST- 1983/10/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1983 Oct;131(4):2050-8.