PMID- 6194697
OWN - NLM
STAT- MEDLINE
DCOM- 19831123
LR  - 20181130
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 245
IP  - 4
DP  - 1983 Oct
TI  - Cl- requirement of acid secretion in isolated gastric glands.
PG  - G573-81
AB  - The dependence of acid formation, as measured by aminopyrine (AP) accumulation, 
      on medium and intracellular Cl- was investigated in resting (10(-4) M cimetidine) 
      and stimulated (10(-3) M dibutyryl cAMP) gastric glands isolated from the rabbit 
      stomach. Intracellular Cl- concentrations (Cli-) were measured by the 
      steady-state distribution of 36Cl-. In Cl- -free conditions AP accumulation was 
      absent. Medium Cl- induced AP accumulation (AP ratio = 125) in stimulated glands 
      with a K0.5 of 10.4 +/- 1.1 mM, equivalent to 18.0 +/- 1.2 mM Cli-, and had a 
      small effect on resting glands (AP ratio = 6). With normal Nai+ and Ki+ 
      maintained, similar results were obtained in stimulated glands treated with 
      10(-5) M amphotericin (ampho) and 10(-4) M ouabain (ouab), where the 
      basal-lateral membrane was confirmed to be short-circuited with respect to Cl- 
      pathways, i.e., medium Cl- and Cli- were equal. The K0.5 for Cl-i was 17.5 +/- 
      2.5 mM. In resting glands treated with ampho and ouab, AP accumulation increased 
      linearly (no saturation was observed) with increasing Cl- (AP ratio = 35). These 
      results suggest that stimulation activates a Cl- component in the secretory 
      membrane and not in the basal-lateral membrane of the parietal cell. The K+ 
      requirement of AP accumulation at a physiological Cl-i of 60 mM was also 
      investigated in ampho- and ouab-treated glands. On stimulation the K0.5 for Ki+ 
      decreased from 19.5 to 12 mM, coupled with a large increase in AP accumulation, 
      indicating that stimulation also activates a K+ component in the secretory 
      membrane.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Malinowska, D H
AU  - Malinowska DH
FAU - Cuppoletti, J
AU  - Cuppoletti J
FAU - Sachs, G
AU  - Sachs G
LA  - eng
GR  - AM-27606/AM/NIADDK NIH HHS/United States
GR  - AM-28459/AM/NIADDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Chlorides)
RN  - 01704YP3MO (Aminopyrine)
RN  - 5ACL011P69 (Ouabain)
RN  - 63X7MBT2LQ (Bucladesine)
RN  - 7XU7A7DROE (Amphotericin B)
RN  - 820484N8I3 (Histamine)
RN  - RWP5GA015D (Potassium)
RN  - TBT296U68M (1-Methyl-3-isobutylxanthine)
SB  - IM
MH  - 1-Methyl-3-isobutylxanthine/pharmacology
MH  - Aminopyrine/metabolism
MH  - Amphotericin B/pharmacology
MH  - Animals
MH  - Bucladesine/pharmacology
MH  - Chlorides/*metabolism
MH  - Gastric Juice/drug effects/*metabolism
MH  - Gastric Mucosa/drug effects/*metabolism
MH  - Histamine/pharmacology
MH  - Kinetics
MH  - Ouabain/pharmacology
MH  - Potassium/pharmacology
MH  - Rabbits
EDAT- 1983/10/01 00:00
MHDA- 1983/10/01 00:01
CRDT- 1983/10/01 00:00
PHST- 1983/10/01 00:00 [pubmed]
PHST- 1983/10/01 00:01 [medline]
PHST- 1983/10/01 00:00 [entrez]
AID - 10.1152/ajpgi.1983.245.4.G573 [doi]
PST - ppublish
SO  - Am J Physiol. 1983 Oct;245(4):G573-81. doi: 10.1152/ajpgi.1983.245.4.G573.