PMID- 6197177
OWN - NLM
STAT- MEDLINE
DCOM- 19840214
LR  - 20181113
IS  - 0163-4992 (Print)
IS  - 0163-4992 (Linking)
VI  - 5
IP  - 2
DP  - 1983 Jun
TI  - The G1 period. Two cycles of chromatin conformational changes monitored by single 
      cell dye intercalation.
PG  - 79-94
AB  - In previous papers the existence of two cycles of chromosome 
      condensation-decondensation per cell cycle was suggested based on experiments 
      involving nuclear morphometry measurements of Feulgen-stained nuclei. This 
      conclusion can be criticized since its assumption of a relationship between 
      nuclear morphology and chromatin structure is derived from indirect evidence. In 
      this paper, we report simultaneous measurements of nuclear area and nuclear 
      fluorescence intensity on individual cells stained with the intercalating dye, 
      acridine orange (AO). Using cells in various stages of G1 and synchronized by two 
      different methods, our results demonstrate a linear correlation between nuclear 
      area and fluorescence intensity. They also indicate two cycles of chromatin 
      condensation-decondensation during the G1 period, as assayed by the number of 
      chromatin primary, intercalating AO binding sites. Finally, they show that the 
      first of these cycles involves a transition in early G1 from a very small 
      condensed nucleus (immediately after telephase) to a relatively large, dispersed 
      nucleus that occurs abruptly.
FAU - Belmont, A
AU  - Belmont A
FAU - Nicolini, C
AU  - Nicolini C
LA  - eng
GR  - CA20034/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell Biophys
JT  - Cell biophysics
JID - 8002185
RN  - 0 (Butyrates)
RN  - 0 (Chromatin)
RN  - 107-92-6 (Butyric Acid)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Butyrates
MH  - Butyric Acid
MH  - Cell Line
MH  - Chromatin/*ultrastructure
MH  - DNA/analysis
MH  - Flow Cytometry
MH  - Fluorometry
MH  - Humans
MH  - *Interphase
MH  - *Nucleic Acid Conformation
EDAT- 1983/06/01 00:00
MHDA- 1983/06/01 00:01
CRDT- 1983/06/01 00:00
PHST- 1983/06/01 00:00 [pubmed]
PHST- 1983/06/01 00:01 [medline]
PHST- 1983/06/01 00:00 [entrez]
AID - 10.1007/BF02796135 [doi]
PST - ppublish
SO  - Cell Biophys. 1983 Jun;5(2):79-94. doi: 10.1007/BF02796135.