PMID- 6197440
OWN - NLM
STAT- MEDLINE
DCOM- 19840214
LR  - 20170214
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 32
IP  - 1
DP  - 1984 Jan
TI  - Use of acridine orange for histologic analysis of the central nervous system.
PG  - 97-104
AB  - The application of the fluorescent dye acridine orange (AO) to the staining of 
      histologic sections of the brain, and its use for automatic cyto- and 
      histophotometric evaluation are described and compared with the results obtained 
      using cresyl violet. The most suitable procedure for aldehyde-fixed brain tissue, 
      embedded in paraffin and sectioned at 5 micron, proved to be treatment of the 
      sections with an aqueous solution of AO (1:50,000) at pH 1.2 for 30 min, followed 
      by rinsing in distilled water for 10 min. This procedure revealed the morphology 
      in a highly acceptable manner, clearly differentiating various cell components; 
      its characteristics included exact reproducibility and high contrast. The degree 
      of fading was calculable, with a very gradual decrease in fluorescent intensity. 
      The AO procedure appears to be compatible with most other staining procedures 
      that do not rely on the same binding mechanisms. Thus, AO staining has two 
      advantages over the classical cytoarchitectural staining methods: first, it is 
      more suitable for automated morphometric analysis, and second, it can be used in 
      conjunction with immunologic and other techniques.
FAU - Mai, J K
AU  - Mai JK
FAU - Schmidt-Kastner, R
AU  - Schmidt-Kastner R
FAU - Tefett, H B
AU  - Tefett HB
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Animals
MH  - Brain/*cytology
MH  - Histological Techniques
MH  - Male
MH  - Photometry
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Staining and Labeling
EDAT- 1984/01/01 00:00
MHDA- 1984/01/01 00:01
CRDT- 1984/01/01 00:00
PHST- 1984/01/01 00:00 [pubmed]
PHST- 1984/01/01 00:01 [medline]
PHST- 1984/01/01 00:00 [entrez]
AID - 10.1177/32.1.6197440 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 1984 Jan;32(1):97-104. doi: 10.1177/32.1.6197440.