PMID- 6197975
OWN - NLM
STAT- MEDLINE
DCOM- 19840214
LR  - 20190623
IS  - 0006-2952 (Print)
IS  - 0006-2952 (Linking)
VI  - 32
IP  - 24
DP  - 1983 Dec 15
TI  - Interactions of acridine orange with nucleic acids. Properties of complexes of 
      acridine orange with single stranded ribonucleic acid.
PG  - 3679-94
AB  - Interactions between acridine orange (AO) and nucleic acids (calf thymus DNA, and 
      homoribo- and homodeoxyribo-polynucleotides) were studied in solutions containing 
      ethanol as a cosolvent. Light absorption, scattering and luminescence were 
      measured as a function of AO concentration at different dye/phosphate (D/P) 
      ratios, and the data were analyzed using the McGhee-von Hippel probabilistic 
      model of the polymer-ligand interactions. The absorption spectra of AO complexes 
      with four homoribopolymers are presented. The intrinsic association constants and 
      cooperativity coefficients of the formation of the complexes were calculated. The 
      effects of ethanol (up to 35%, v/v) on these interactions were concentration 
      dependent and may be extrapolated to zero concentration of this cosolvent. The 
      possibility of destabilization of the double helix of nucleic acids by AO at high 
      D/P ratios is discussed in light of the available thermodynamic data.
FAU - Kapuscinski, J
AU  - Kapuscinski J
FAU - Darzynkiewicz, Z
AU  - Darzynkiewicz Z
FAU - Melamed, M R
AU  - Melamed MR
LA  - eng
GR  - CA-14134/CA/NCI NIH HHS/United States
GR  - CA-232-96/CA/NCI NIH HHS/United States
GR  - CA-28704/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Biochem Pharmacol
JT  - Biochemical pharmacology
JID - 0101032
RN  - 3K9958V90M (Ethanol)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*pharmacology
MH  - Animals
MH  - Binding Sites/drug effects
MH  - Cattle
MH  - Chemical Phenomena
MH  - Chemistry
MH  - DNA/*analysis
MH  - Ethanol
MH  - Light
MH  - Models, Chemical
MH  - RNA/*analysis
MH  - Scattering, Radiation
MH  - Spectrometry, Fluorescence
MH  - Spectrophotometry
MH  - Thymus Gland/metabolism
EDAT- 1983/12/15 00:00
MHDA- 1983/12/15 00:01
CRDT- 1983/12/15 00:00
PHST- 1983/12/15 00:00 [pubmed]
PHST- 1983/12/15 00:01 [medline]
PHST- 1983/12/15 00:00 [entrez]
AID - 0006-2952(83)90136-3 [pii]
AID - 10.1016/0006-2952(83)90136-3 [doi]
PST - ppublish
SO  - Biochem Pharmacol. 1983 Dec 15;32(24):3679-94. doi: 10.1016/0006-2952(83)90136-3.