PMID- 6199138
OWN - NLM
STAT- MEDLINE
DCOM- 19840330
LR  - 20181113
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 55
IP  - 2
DP  - 1984 Feb
TI  - Flare-up of antigen-induced arthritis in mice after challenge with intravenous 
      antigen: studies on the characteristics of and mechanisms involved in the 
      reaction.
PG  - 287-94
AB  - Intravenous (i.v.) administration of 300 micrograms methylated BSA (mBSA) to 
      C57-Black mice with unilateral antigen-induced arthritis (AIA) of 4-6 weeks 
      duration resulted in a reproducible flare-up of ongoing arthritis without signs 
      of inflammation in the contralateral non-arthritic knee joint. In the present 
      study we investigated characteristics of the flare-up phenomenon using 
      histological grading and 99mtechnetium pertechnetate uptake measurements to 
      detect and quantitate knee joint inflammation. At 6 h after intravenous mBSA 
      administration flare-up of arthritis was demonstrable, showing histological 
      characteristics of acute joint inflammation; later this progressed to chronic 
      stages, but the inflammatory stage did not return to baseline values up to day 9 
      after i.v. challenge. Varying the dose of i.v. mBSA from 2 to 1000 micrograms 
      showed that 10 micrograms was sufficient to elicit the phenomenon and also that 
      the latter is dose-dependent. Using mice immunized with both mBSA and methylated 
      HGG (mHGG) with either mBSA or mHGG-induced arthritis, we could show that 4-6 
      weeks after arthritis induction the flare-up phenomenon is antigen specific. 
      Intra-articular injection of as little as 10 ng of mBSA resulted in exacerbation 
      of inflammation in joints with chronic mBSA-induced arthritis, but induced no 
      inflammation in the contralateral non-arthritic knee joints, indicating local 
      hypersensitivity in the former joints. The flare-up phenomenon may be due to 
      local hyper-reactivity of chronically inflamed joints to minimal amounts of 
      circulating antigen entering the joint.
FAU - Lens, J W
AU  - Lens JW
FAU - van den Berg, W B
AU  - van den Berg WB
FAU - van de Putte, L B
AU  - van de Putte LB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Antigens)
RN  - 0 (Epitopes)
RN  - 0 (gamma-Globulins)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 7440-26-8 (Technetium)
RN  - A0730CX801 (Sodium Pertechnetate Tc 99m)
SB  - IM
MH  - Animals
MH  - Antigens/*immunology
MH  - Arthritis/*immunology
MH  - Arthritis, Experimental/*immunology/pathology
MH  - Dose-Response Relationship, Immunologic
MH  - Epitopes
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Serum Albumin, Bovine/immunology
MH  - Sodium Pertechnetate Tc 99m
MH  - Synovial Membrane/pathology
MH  - Technetium
MH  - Time Factors
MH  - gamma-Globulins/immunology
PMC - PMC1535823
EDAT- 1984/02/01 00:00
MHDA- 1984/02/01 00:01
PMCR- 1985/02/01
CRDT- 1984/02/01 00:00
PHST- 1984/02/01 00:00 [pubmed]
PHST- 1984/02/01 00:01 [medline]
PHST- 1984/02/01 00:00 [entrez]
PHST- 1985/02/01 00:00 [pmc-release]
PST - ppublish
SO  - Clin Exp Immunol. 1984 Feb;55(2):287-94.