PMID- 6200702
OWN - NLM
STAT- MEDLINE
DCOM- 19840517
LR  - 20190817
IS  - 0022-4731 (Print)
IS  - 0022-4731 (Linking)
VI  - 20
IP  - 1
DP  - 1984 Jan
TI  - Glucocorticoid hormone interactions with cloned proviral DNA of mouse mammary 
      tumor virus.
PG  - 95-8
AB  - The molecular details of glucocorticoid hormone regulation of expression of the 
      mouse mammary tumor virus (MMTV) proviral gene have been investigated. Cloned 
      proviral DNA was introduced into cultured cells by a gene transfer procedure. DNA 
      acquired by transfection was shown to be expressed in a hormone regulated 
      fashion. The proviral DNA was fragmented and recombined in vitro with an 
      indicator gene to delimit the hormone response sequence. Inducibility of the 
      indicator gene (thymidine kinase gene from Herpes Simplex Virus, tk) was observed 
      upon recombination with the long terminal repeat (LTR) sequence of MMTV. Further 
      delimitation of the LTR DNA demonstrated that 202 nucleotides located 5' of the 
      RNA initiation site are sufficient to confer glucocorticoid regulation. In vitro 
      interaction of LTR DNA with glucocorticoid hormone receptor complex, showed a 
      preferential affinity to the same sequence which mediated hormonal regulation in 
      transfected cells. Evidence for a direct receptor gene interaction in the process 
      of gene induction was gained by the measurement of the kinetics of induction and 
      the use of a glucocorticoid antagonist (RU 486). The induction of the transfected 
      gene is very rapid, independent of simultaneous protein synthesis and requires a 
      functional glucocorticoid receptor hormone complex.
FAU - Groner, B
AU  - Groner B
FAU - Ponta, H
AU  - Ponta H
FAU - Rahmsdorf, U
AU  - Rahmsdorf U
FAU - Herrlich, P
AU  - Herrlich P
FAU - Pfahl, M
AU  - Pfahl M
FAU - Hynes, N E
AU  - Hynes NE
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Steroid Biochem
JT  - Journal of steroid biochemistry
JID - 0260125
RN  - 0 (DNA, Viral)
RN  - 0 (Glucocorticoids)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Viral/*metabolism
MH  - Glucocorticoids/*metabolism
MH  - *Lysogeny
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mice
MH  - RNA/metabolism
MH  - Receptors, Glucocorticoid/metabolism
MH  - Repetitive Sequences, Nucleic Acid
EDAT- 1984/01/01 00:00
MHDA- 1984/01/01 00:01
CRDT- 1984/01/01 00:00
PHST- 1984/01/01 00:00 [pubmed]
PHST- 1984/01/01 00:01 [medline]
PHST- 1984/01/01 00:00 [entrez]
AID - 10.1016/0022-4731(84)90194-8 [doi]
PST - ppublish
SO  - J Steroid Biochem. 1984 Jan;20(1):95-8. doi: 10.1016/0022-4731(84)90194-8.