PMID- 6202514
OWN - NLM
STAT- MEDLINE
DCOM- 19840709
LR  - 20220511
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 3
IP  - 4
DP  - 1984 Apr
TI  - Selective amplification in methotrexate-resistant mouse cells of an artificial 
      dihydrofolate reductase transcription unit making use of cryptic splicing and 
      polyadenylation sites.
PG  - 901-8
AB  - We have constructed a recombinant ( pMOP ) which is based on the bacterial 
      plasmid pML2 and contains bovine papilloma virus type 1 (BPV 1) sequences linked 
      to an artificial mouse dihydrofolate reductase (DHFR) transcription unit. This 
      unit consists of the SV40 early gene promoter, a complete DHFR coding sequence 
      and splice and polyadenylation sites from a rabbit beta-globin gene. Intact pMOP 
      or a fragment thereof devoid of pML2 sequences will transform mouse cells to 
      methotrexate resistance. The lines obtained contain approximately 200 copies of 
      the DHFR transcription unit. In no case, however, did we find evidence of 
      extrachromosomal maintenance of BPV 1 or DHFR sequences. One line, when selected 
      for resistance to elevated levels of methotrexate, contained amplified copies of 
      a 'novel' DHFR transcription unit resulting from fusion of two normal units. This 
      line contained the DHFR RNA species present in the parent line plus some 
      additional species. The structure of these various RNA species was determined and 
      evidence found for the use of cryptic splice and polyadenylation sites.
FAU - Breathnach, R
AU  - Breathnach R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (DNA, Recombinant)
RN  - 24937-83-5 (Poly A)
RN  - 63231-63-0 (RNA)
RN  - 9004-22-2 (Globins)
RN  - EC 1.5.1.3 (Tetrahydrofolate Dehydrogenase)
RN  - EC 3.1.21.- (DNA Restriction Enzymes)
RN  - YL5FZ2Y5U1 (Methotrexate)
SB  - IM
MH  - Animals
MH  - Bovine papillomavirus 1/*genetics
MH  - Cell Line
MH  - Cell Transformation, Viral
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - DNA Restriction Enzymes
MH  - *DNA, Recombinant
MH  - Drug Resistance
MH  - Fibroblasts/enzymology
MH  - *Gene Amplification
MH  - Globins/genetics
MH  - Methotrexate/*pharmacology
MH  - Mice
MH  - Papillomaviridae/*genetics
MH  - Plasmids
MH  - Poly A/metabolism
MH  - RNA
MH  - Tetrahydrofolate Dehydrogenase/*genetics
MH  - Transcription, Genetic
PMC - PMC557445
EDAT- 1984/04/01 00:00
MHDA- 1984/04/01 00:01
PMCR- 1985/04/01
CRDT- 1984/04/01 00:00
PHST- 1984/04/01 00:00 [pubmed]
PHST- 1984/04/01 00:01 [medline]
PHST- 1984/04/01 00:00 [entrez]
PHST- 1985/04/01 00:00 [pmc-release]
AID - 10.1002/j.1460-2075.1984.tb01903.x [doi]
PST - ppublish
SO  - EMBO J. 1984 Apr;3(4):901-8. doi: 10.1002/j.1460-2075.1984.tb01903.x.