PMID- 6205072
OWN - NLM
STAT- MEDLINE
DCOM- 19840918
LR  - 20061115
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 133
IP  - 3
DP  - 1984 Sep
TI  - Human T cell clones exerting multiple cytolytic activities show heterogeneity in 
      susceptibility to inhibition by monoclonal antibodies.
PG  - 1222-9
AB  - Human cytotoxic T cell clones (CTL) were obtained by limiting dilution after in 
      vitro priming against an allogeneic Epstein Barr virus (EBV)-transformed B cell 
      line (B-LCL) BSM. Three OKT3+, OKT8+ E rosette-forming (RFC) but EA gamma-RFC- 
      clones with cytotoxic activity against the stimulator cell and one 
      "non-cytolytic" clone were expanded for over 50 generations and further 
      characterized. Clone G9 showed allospecific lysis of Cw3+ lymphocytes and B cell 
      lines. Three cytolytic clones (G9, D11, and A3) showed cytotoxicity to the 
      stimulator B-LCL, to the human plasma cell leukemia-derived line LICR-LON-HMY2 
      and to short-term cultured melanoma cells (O-mel). Four other EBV-transformed 
      B-LCL unrelated to the stimulator B-LCL were not lysed. These clones also exerted 
      cytotoxic activity against NK-sensitive target cells (TC), e.g., the 
      erythroleukemia cell line K562. Other NK-sensitive TC, e.g., lymphoma-derived 
      Daudi cells, were killed provided they were pretreated with phytohemagglutinin 
      (PHA). Cytolytic activity against the B-LCL cell LICR-LON and O-mel, but not 
      against K562 or PHA-treated target cells, was inhibited by monoclonal anti-HLA 
      ABC antibodies (MCA). The cytolytic activities of OKT3+,8+ clones G9 and A3 but 
      not that of OKT3+,8+ clone D11 were inhibited by OKT8. Another MCA, 13.3, 
      directed against the murine glycoprotein T-200, inhibited the cytolytic activity 
      of clone D11 against K562 but not against the stimulator cells. Clone G9 was not 
      inhibited by MCA 13.3. The four clones, including the OKT4+ "non-cytotoxic" clone 
      K12, exerted lytic activity against TC that are normally resistant to lysis 
      provided these TC were pretreated with PHA. The TC specificity range of the 
      clones was confirmed by cold target inhibition experiments. A correlation between 
      blocking of lytic activity by cold TC and the percentage of conjugate formation 
      with the particular cold TC was observed. Because these clones also show 
      differential susceptibility to inhibition of lysis by various MCA, it is 
      concluded that human cytotoxic T cell clones can exert multiple lytic activities, 
      i.e., the operationally defined lytic mechanisms differ at least at certain 
      stages of the lytic cycle.
FAU - van de Griend, R J
AU  - van de Griend RJ
FAU - Giphart, M J
AU  - Giphart MJ
FAU - Van Krimpen, B A
AU  - Van Krimpen BA
FAU - Bolhuis, R L
AU  - Bolhuis RL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Surface)
RN  - 0 (Epitopes)
RN  - 0 (HLA Antigens)
RN  - 0 (Phytohemagglutinins)
SB  - IM
MH  - Antibodies, Monoclonal/*physiology
MH  - Antigens, Surface/immunology
MH  - Binding, Competitive
MH  - Clone Cells/classification/immunology
MH  - *Cytotoxicity, Immunologic
MH  - Epitopes
MH  - HLA Antigens/immunology
MH  - Humans
MH  - Lymphocyte Activation
MH  - Phenotype
MH  - Phytohemagglutinins/pharmacology
MH  - T-Lymphocytes, Cytotoxic/classification/*immunology
EDAT- 1984/09/01 00:00
MHDA- 1984/09/01 00:01
CRDT- 1984/09/01 00:00
PHST- 1984/09/01 00:00 [pubmed]
PHST- 1984/09/01 00:01 [medline]
PHST- 1984/09/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1984 Sep;133(3):1222-9.