PMID- 6205708
OWN - NLM
STAT- MEDLINE
DCOM- 19841001
LR  - 20190904
IS  - 0006-5242 (Print)
IS  - 0006-5242 (Linking)
VI  - 49
IP  - 2
DP  - 1984 Aug
TI  - A new method for fast blood cell counting and partial differentiation by flow 
      cytometry.
PG  - 83-90
AB  - A new blood counting method by flow cytometry is described which determines 
      absolute counts and relative proportions of erythrocytes, reticulocytes, 
      thrombocytes, lymphocytes and granulocytes from one sample of saline diluted 
      human or animal blood. Staining time is 2 to 5 min and measuring time between 1 
      and 2 additional minutes. Measured simultaneously are the electrical cell volume, 
      the green and optionally also the red fluorescence of the transmembrane potential 
      sensitive dye 3,3-dihexyloxacarbocyanine DiOC6(3) and the RNA/DNA stain acridine 
      orange (AO). Work is under way to fully automate staining, measurement and data 
      evaluation. The use of stains by which blood cell counting and biochemical 
      analysis can be combined offers new possibilities for routine blood cell counting 
      without requirement for additional time. The potential of such stains is that 
      pathologic cell conditions which are not, or not yet reflected in the cell count 
      may be earlier detectable by biochemical stains.
FAU - Valet, G
AU  - Valet G
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Blut
JT  - Blut
JID - 0173401
RN  - 0 (Fluorescent Dyes)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Adult
MH  - Blood Cell Count/*methods
MH  - Erythrocyte Count
MH  - Erythrocyte Volume
MH  - Flow Cytometry
MH  - Fluorescent Dyes
MH  - Humans
MH  - Leukocyte Count
MH  - Platelet Count
MH  - Reticulocytes
MH  - Staining and Labeling
MH  - Time Factors
EDAT- 1984/08/01 00:00
MHDA- 1984/08/01 00:01
CRDT- 1984/08/01 00:00
PHST- 1984/08/01 00:00 [pubmed]
PHST- 1984/08/01 00:01 [medline]
PHST- 1984/08/01 00:00 [entrez]
AID - 10.1007/BF00363698 [doi]
PST - ppublish
SO  - Blut. 1984 Aug;49(2):83-90. doi: 10.1007/BF00363698.