PMID- 6205774
OWN - NLM
STAT- MEDLINE
DCOM- 19841017
LR  - 20190720
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 87
IP  - 2
DP  - 1984 Sep
TI  - Macrophage-activating factor for cytotoxicity produced by a human T-cell 
      hybridoma.
PG  - 626-36
AB  - Human MAF-C (macrophage-activation factor for cytotoxicity)-producing hybridoma 
      H2-E3-5 was prepared by somatic cell fusion of PHA-activated peripheral blood 
      lymphocytes with emetine/actinomycin D-treated cloned human acute lymphatic 
      leukemia cells (CEM). The following activities were assayed: (1) 
      macrophage-migration-inhibitory factor (MIF), (2) macrophage-activation factor 
      for glucose consumption (MAF-G), (3) macrophage-activation factor for 
      O2-formation (MAF-O), and (4) macrophage-activation factor for cytotoxicity 
      (MAF-C). After anion-exchange chromatography, MAF-C activity could be 
      distinguished from MIF and MAF-O activities. It is shown that MAF-C is not the 
      same as MAF-G from the culture supernatants of CEM 11, a parent cell line of 
      H2-E3-5. Furthermore, MAF-C from H2-E3-5 culture supernatants activated 
      differentiated macrophages but not monocytes.
FAU - Higuchi, M
AU  - Higuchi M
FAU - Nakamura, N
AU  - Nakamura N
FAU - Tsuchiya, S I
AU  - Tsuchiya SI
FAU - Kobayashi, Y
AU  - Kobayashi Y
FAU - Osawa, T
AU  - Osawa T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Antigens, Surface)
RN  - 0 (Lymphokines)
RN  - 0 (Macrophage-Activating Factors)
RN  - 9008-11-1 (Interferons)
SB  - IM
MH  - Antigens, Surface/analysis
MH  - *Cytotoxicity, Immunologic
MH  - Humans
MH  - Hybridomas/*immunology
MH  - Immunity, Cellular
MH  - Interferons/immunology
MH  - Lymphokines/*biosynthesis
MH  - Macrophage-Activating Factors
MH  - Macrophages/*immunology
MH  - Monocytes/immunology
MH  - Neoplasms, Experimental/immunology
MH  - T-Lymphocytes/*immunology/metabolism
EDAT- 1984/09/01 00:00
MHDA- 1984/09/01 00:01
CRDT- 1984/09/01 00:00
PHST- 1984/09/01 00:00 [pubmed]
PHST- 1984/09/01 00:01 [medline]
PHST- 1984/09/01 00:00 [entrez]
AID - 0008-8749(84)90030-3 [pii]
AID - 10.1016/0008-8749(84)90030-3 [doi]
PST - ppublish
SO  - Cell Immunol. 1984 Sep;87(2):626-36. doi: 10.1016/0008-8749(84)90030-3.