PMID- 6205888
OWN - NLM
STAT- MEDLINE
DCOM- 19841024
LR  - 20190707
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 154
IP  - 1
DP  - 1984 Sep
TI  - Cellular content of ribosomal RNA in relation to the progression and competence 
      signals governing proliferation of 3T3 and SV40-3T3 cells.
PG  - 10-24
AB  - The method for differential fluorescence staining of cellular RNA and DNA by 
      acridine orange (AO) was optimized for 3T3 and SV40-3T3 cells. Cellular contents 
      of DNA and of ribosomal RNA (rRNA) were determined by dual-channel flow cytometry 
      during cell-density-dependent proliferation and after stimulation of quiescent 
      cells. With increasing density of 3T3 cells, cellular content of rRNA decreases 
      by about 60%, whereas SV40-3T3 cells do not exhibit a significant dependence of 
      rRNA content on cell density. 3T3 cells stimulated early after becoming quiescent 
      resume reaccumulation of rRNA after a delay of only 4 h, whereas cells maintained 
      at quiescence for several days exhibit a delay of about 12 h before a significant 
      rise of rRNA is observed. The extent of rise of cellular rRNA content after 
      different regimens of stimulation of quiescent 3T3 cells does not correlate well 
      with the fraction of cells entering the cell cycle. These and other reported 
      instances of discordance between rRNA content and stimulation into the cell cycle 
      are resolved by showing that of the two signals governing entry into the cell 
      cycle only the progression signal, but not the competence signal is associated 
      with reaccumulation of cellular rRNA. The present results are consistent with the 
      progression function being in essence the achievement of a threshold number of 
      ribosomes per cell, which in conjunction with the competence signal is sufficient 
      for initiation of the cell cycle.
FAU - Seuwen, K
AU  - Seuwen K
FAU - Steiner, U
AU  - Steiner U
FAU - Adam, G
AU  - Adam G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (RNA, Ribosomal)
SB  - IM
MH  - Animals
MH  - *Cell Cycle
MH  - Cell Division
MH  - Cell Nucleus/physiology
MH  - *Cell Transformation, Viral
MH  - Cells, Cultured
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - RNA, Ribosomal/*metabolism
MH  - Simian virus 40/*genetics
MH  - Staining and Labeling
EDAT- 1984/09/01 00:00
MHDA- 1984/09/01 00:01
CRDT- 1984/09/01 00:00
PHST- 1984/09/01 00:00 [pubmed]
PHST- 1984/09/01 00:01 [medline]
PHST- 1984/09/01 00:00 [entrez]
AID - 0014-4827(84)90664-5 [pii]
AID - 10.1016/0014-4827(84)90664-5 [doi]
PST - ppublish
SO  - Exp Cell Res. 1984 Sep;154(1):10-24. doi: 10.1016/0014-4827(84)90664-5.