PMID- 6209687
OWN - NLM
STAT- MEDLINE
DCOM- 19850118
LR  - 20190818
IS  - 0196-9781 (Print)
IS  - 0196-9781 (Linking)
VI  - 5
IP  - 5
DP  - 1984 Sep-Oct
TI  - Neuropeptidergic inhibitory regulation of Met-enkephalin immunoreactive material 
      release from rat spinal cord in vitro.
PG  - 849-52
AB  - The release of Met-enkephalin immunoreactive material (ME-IR) from rat spinal 
      slices was measured in vitro. This release increased about 4 fold in response to 
      the addition of K+ ions. K+-evoked release of ME-IR was Ca++ dependent. 
      Veratridine, a depolarizing agent, also stimulated the release of ME-IR. 
      Veratridine-induced ME-IR release was completely prevented by tetrodotoxin (TTX), 
      a Na+ channel blocker. Somatostatin (SRIF) inhibited both basal and K+-evoked 
      release of ME-IR at 10(-7) M. Substance P had a similar effect although higher 
      concentrations were needed. gamma-Aminobutyric acid (GABA) and neurotensin (NT) 
      did not affect the basal release but slightly decreased K+-evoked release at 
      10(-5) M. Serotonin (5-HT) and noradrenaline (NA), did not affect ME-IR release. 
      These results suggest that some of the neuropeptides present in the spinal cord, 
      especially SP and SRIF, may be potent modulators of ME-IR release at the spinal 
      level.
FAU - Uzumaki, U
AU  - Uzumaki U
FAU - Govoni, S
AU  - Govoni S
FAU - Faccini, E
AU  - Faccini E
FAU - Pasinetti, G
AU  - Pasinetti G
FAU - Missale, C
AU  - Missale C
FAU - Trabucchi, M
AU  - Trabucchi M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Peptides
JT  - Peptides
JID - 8008690
RN  - 0 (Neurotransmitter Agents)
RN  - 333DO1RDJY (Serotonin)
RN  - 33507-63-0 (Substance P)
RN  - 39379-15-2 (Neurotensin)
RN  - 4368-28-9 (Tetrodotoxin)
RN  - 51110-01-1 (Somatostatin)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - 58569-55-4 (Enkephalin, Methionine)
RN  - 71-62-5 (Veratridine)
RN  - RWP5GA015D (Potassium)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - Animals
MH  - Enkephalin, Methionine/*metabolism
MH  - Male
MH  - Neurotensin/pharmacology
MH  - Neurotransmitter Agents/*pharmacology
MH  - Norepinephrine/pharmacology
MH  - Potassium/pharmacology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Serotonin/pharmacology
MH  - Somatostatin/pharmacology
MH  - Spinal Cord/drug effects/*metabolism
MH  - Substance P/pharmacology
MH  - Tetrodotoxin/pharmacology
MH  - Veratridine/pharmacology
MH  - gamma-Aminobutyric Acid/pharmacology
EDAT- 1984/09/01 00:00
MHDA- 1984/09/01 00:01
CRDT- 1984/09/01 00:00
PHST- 1984/09/01 00:00 [pubmed]
PHST- 1984/09/01 00:01 [medline]
PHST- 1984/09/01 00:00 [entrez]
AID - 0196-9781(84)90104-9 [pii]
AID - 10.1016/0196-9781(84)90104-9 [doi]
PST - ppublish
SO  - Peptides. 1984 Sep-Oct;5(5):849-52. doi: 10.1016/0196-9781(84)90104-9.