PMID- 6212079
OWN - NLM
STAT- MEDLINE
DCOM- 19820910
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 21
IP  - 9
DP  - 1982 Apr 27
TI  - Phosphorylation of the receptor of immunoglobulin E.
PG  - 2004-10
AB  - Specific immune precipitation of immunoglobulin E(IgE)-receptor complexes from 
      detergent extracts of 32P-labeled rat basophilic leukemia cells yielded a 
      phosphoprotein of Mr approximately 35,000 on gel electrophoresis in sodium 
      dodecyl sulfate. This phosphoprotein was shown by several criteria to be the beta 
      chain of the receptor for IgE. Phosphorylation occurs at a serine residue (or 
      residues) in a region (beta 2) of the beta chain that is thought to be exposed on 
      the cytoplasmic face of the plasma membrane. Our results suggest that 
      phosphorylation probably takes place after the insertion of the beta chain into 
      the membrane. The IgE-binding alpha chain of the receptor and the IgE associated 
      with it are not phosphorylated. We have so far been unable to detect any changes 
      in the state of phosphorylation of either chain of the receptor or of IgE itself 
      after IgE-mediated triggering of the cells.
FAU - Fewtrell, C
AU  - Fewtrell C
FAU - Davis, C L
AU  - Davis CL
FAU - Metzger, H
AU  - Metzger H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Phosphoproteins)
RN  - 0 (Receptors, IgE)
RN  - 0 (Receptors, Immunologic)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Basophils
MH  - Cells, Cultured
MH  - Cross-Linking Reagents/pharmacology
MH  - *Immunoglobulin E
MH  - Leukemia, Experimental/immunology
MH  - Molecular Weight
MH  - Phosphoproteins/immunology
MH  - Phosphorylation
MH  - Rats
MH  - Receptors, IgE
MH  - Receptors, Immunologic/*metabolism
EDAT- 1982/04/27 00:00
MHDA- 1982/04/27 00:01
CRDT- 1982/04/27 00:00
PHST- 1982/04/27 00:00 [pubmed]
PHST- 1982/04/27 00:01 [medline]
PHST- 1982/04/27 00:00 [entrez]
AID - 10.1021/bi00538a005 [doi]
PST - ppublish
SO  - Biochemistry. 1982 Apr 27;21(9):2004-10. doi: 10.1021/bi00538a005.