PMID- 6212760
OWN - NLM
STAT- MEDLINE
DCOM- 19820910
LR  - 20190825
IS  - 0161-5890 (Print)
IS  - 0161-5890 (Linking)
VI  - 19
IP  - 2
DP  - 1982 Feb
TI  - Further characterization of the beta-component of the receptor for immunoglobulin 
      E.
PG  - 219-27
AB  - A 30,000 mol. wt component (beta) is associated in a 1:1 ratio with the 50,000 
      mol. wt glycoprotein (alpha) which binds immunoglobulin E (IgE) on mast cells and 
      related tumor cells. We show that alpha and beta are associated in membrane 
      preparations. This is consistent with previous results which showed labeling of 
      beta with the hydrophobic probe 5-iodonaphthyl-1-azide (INA). The 
      beta-polypeptide is susceptible to proteolytic cleavage during preparation of the 
      membranes and when this occurs a 20,000 fragment can be labeled with INA and 
      remains associated with alpha. No incorporation of carbohydrate precursors into 
      beta was observed. Since beta is also not modified when cells are 
      surface-labeled, it may not be exposed on the cell surface. Rigorous washing of 
      IgE-receptor complexes with non-ionic detergent results in dissociation of beta 
      from the alpha-IgE complex. The latter will then not reassociate with beta when 
      exposed to crude detergent extracts of the tumor cells.
FAU - Holowka, D
AU  - Holowka D
FAU - Metzger, H
AU  - Metzger H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Membrane Proteins)
RN  - 0 (Receptors, IgE)
RN  - 0 (Receptors, Immunologic)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Humans
MH  - Immunoglobulin E/immunology
MH  - Mast Cells/analysis
MH  - Membrane Proteins/analysis
MH  - Molecular Weight
MH  - Protein Conformation
MH  - Rats
MH  - Receptors, IgE
MH  - Receptors, Immunologic/*analysis/isolation & purification
EDAT- 1982/02/01 00:00
MHDA- 1982/02/01 00:01
CRDT- 1982/02/01 00:00
PHST- 1982/02/01 00:00 [pubmed]
PHST- 1982/02/01 00:01 [medline]
PHST- 1982/02/01 00:00 [entrez]
AID - 10.1016/0161-5890(82)90334-0 [doi]
PST - ppublish
SO  - Mol Immunol. 1982 Feb;19(2):219-27. doi: 10.1016/0161-5890(82)90334-0.