PMID- 6214100
OWN - NLM
STAT- MEDLINE
DCOM- 19821012
LR  - 20190913
IS  - 0044-3255 (Print)
IS  - 0044-3255 (Linking)
VI  - 67
IP  - 2
DP  - 1982
TI  - Isoenzyme studies on cercariae from monoinfections and adult worms of Schistosoma 
      mansoni (10 isolates) and S. rodhaini (one isolate) by horizontal polyacrylamide 
      gel electrophoresis and staining of eight enzymes.
PG  - 185-96
AB  - The enzyme phosphoglucose isomerase (PGI), phosphoglucomutase (PGM), hexokinase 
      (HK), adenylate kinase (AK), fructokinase (FK), mannose-6-phosphate isomerase 
      (MPI), glucose-6-phosphate dehydrogenase (G-6-PDH) and malate dehydrogenase (MDH) 
      were chosen to study the variation between isolates, cercariae and adults, 
      individuals, and sexes of Schistosoma mansoni and S. rodhaini, using horizontal 
      polyacrylamide gel electrophoresis. The method described allows combinations of 
      six of the eight enzymes to be scored in the homogenate from one adult worm. In 
      adult S. mansoni one phenotype of the eight enzymes was observed in all isolates. 
      In addition, the enzyme PGI showed polymorphism in the isolates from Tala, Kenya 
      and Uganda. PGM in the isolates from Tala, Kenya and South Africa showed 
      polymorphism. The cercarial phenotype differs from the adult phenotype in 
      G-6-PDH, where the cercarial enzyme mobility is slower than that in the adult 
      worm. The low amount of intrastrain variation observed in this species is 
      explained by the limited amount of material used to establish the laboratory 
      stocks, whereas the genetic similarity between geographically widely separated 
      stocks does suggest that only limited geographical variation is likely to occur 
      in S. mansoni. It is suggested that the gene controlling the PGI polymorphism is 
      located on the sex chromosomes of S. mansoni. Mobility differences were observed 
      between S. mansoni and S. rodhaini in the enzyme PGI and PGM, and these 
      characteristics might be useful for a quick identification of schistosome 
      cercariae emerging from Biomphalaria sp. in Africa.U
FAU - de Boissezon, B
AU  - de Boissezon B
FAU - Jelnes, J E
AU  - Jelnes JE
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Z Parasitenkd
JT  - Zeitschrift fur Parasitenkunde (Berlin, Germany)
JID - 8710749
RN  - 0 (Isoenzymes)
RN  - EC 1.1.1.37 (Malate Dehydrogenase)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 2.7.1.- (Fructokinases)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.4.3 (Adenylate Kinase)
RN  - EC 5.3.1.8 (Mannose-6-Phosphate Isomerase)
RN  - EC 5.3.1.9 (Glucose-6-Phosphate Isomerase)
RN  - EC 5.4.2.2 (Phosphoglucomutase)
SB  - IM
MH  - Adenylate Kinase
MH  - Animals
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Female
MH  - Fructokinases
MH  - Glucose-6-Phosphate Isomerase
MH  - Glucosephosphate Dehydrogenase
MH  - Hexokinase
MH  - *Isoenzymes
MH  - Malate Dehydrogenase
MH  - Male
MH  - Mannose-6-Phosphate Isomerase
MH  - Phosphoglucomutase
MH  - Schistosoma/classification/*enzymology
MH  - Schistosoma mansoni/classification/*enzymology
EDAT- 1982/01/01 00:00
MHDA- 1982/01/01 00:01
CRDT- 1982/01/01 00:00
PHST- 1982/01/01 00:00 [pubmed]
PHST- 1982/01/01 00:01 [medline]
PHST- 1982/01/01 00:00 [entrez]
AID - 10.1007/BF00928114 [doi]
PST - ppublish
SO  - Z Parasitenkd. 1982;67(2):185-96. doi: 10.1007/BF00928114.