PMID- 6217037
OWN - NLM
STAT- MEDLINE
DCOM- 19830225
LR  - 20190829
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 86
IP  - 3
DP  - 1982
TI  - Dense bodies in silver-stained spermatocytes of the Chinese hamster: behavior and 
      cytochemical nature.
PG  - 359-73
AB  - From the silver staining behavior of various organelles in the nucleus we have 
      divided meiotic prophase (leptotene to the diffuse stage) of the male Chinese 
      hamster into five stages. Components within the nucleus, such as synaptonemal 
      complex (SC), sex bivalent (SB), nucleolus organizer regions (NORs), chromatin 
      and the dense bodies, showed a characteristic feature in each stage of meiotic 
      prophase. The lampbrush chromosome stage was found to be followed by the diffuse 
      stage. The chromatin around SC began to be organized at early pachytene and 
      formed a brush-like structure at late pachytene. During early prophase stages a 
      dramatic change in SB morphology occurred. Three types of morphology of SB were 
      recognized: (1) the XY pair with long synapsis and fusiform or diffuse thickening 
      of the unpaired portions (late zygotene and early pachytene), (2) desynapsed, 
      branched, and anastomosed axes seen at late pachytene. Two types of the dense 
      body were found during meiotic prophase; the double body in early stage 
      (leptotene to early pachytene) and the single body in later stages (mid pachytene 
      to diffuse stage). The small precursors of the double body existed at early 
      leptotene but they increased in size and also changed the silver stainability 
      during zygotene, becoming the characteristic double body consisted of one light 
      body (L-body) and one dark body (D-body). These two bodies can also be recognized 
      after Giemsa or acridine orange (AO) staining. The L-body fluoresced reddish 
      orange after AO staining. The single body, which is probably formed by 
      amalgamation of the D- and the L-bodies, showed a staining reaction similar to 
      that of the D-body. Data from pancreatic lipase and protease treatments suggest 
      that the D-body contained a lipoprotein.
FAU - Takanari, H
AU  - Takanari H
FAU - Pathak, S
AU  - Pathak S
FAU - Hsu, T C
AU  - Hsu TC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
RN  - EC 3.1.- (Endodeoxyribonucleases)
RN  - EC 3.1.- (Ribonucleases)
RN  - EC 3.1.1.3 (Lipase)
RN  - EC 3.1.21.1 (Deoxyribonuclease I)
RN  - EC 3.4.- (Endopeptidases)
RN  - EC 3.4.21.64 (Endopeptidase K)
SB  - IM
MH  - Animals
MH  - Cell Nucleus/*ultrastructure
MH  - Chromosomes/drug effects
MH  - Cricetinae
MH  - Cricetulus
MH  - Deoxyribonuclease I
MH  - Endodeoxyribonucleases/pharmacology
MH  - Endopeptidase K
MH  - Endopeptidases/pharmacology
MH  - Lipase/pharmacology
MH  - Male
MH  - Meiosis
MH  - Organoids/drug effects
MH  - Ribonucleases/pharmacology
MH  - Spermatocytes/*cytology
MH  - Spermatozoa/*cytology
EDAT- 1982/01/01 00:00
MHDA- 1982/01/01 00:01
CRDT- 1982/01/01 00:00
PHST- 1982/01/01 00:00 [pubmed]
PHST- 1982/01/01 00:01 [medline]
PHST- 1982/01/01 00:00 [entrez]
AID - 10.1007/BF00292263 [doi]
PST - ppublish
SO  - Chromosoma. 1982;86(3):359-73. doi: 10.1007/BF00292263.