PMID- 6220739
OWN - NLM
STAT- MEDLINE
DCOM- 19830610
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 22
IP  - 4
DP  - 1983 Feb 15
TI  - Analysis of the rate-limiting step in a ligand-cell receptor interaction: the 
      immunoglobulin E system.
PG  - 954-9
AB  - Theory predicts that the kinetics of simple interactions between a ligand and a 
      receptor bound on the surface of a cell will be affected by the occupancy of 
      receptors on the same cell. In a diffusion-limited reaction the effect will be on 
      the rate of dissociation but not on the rate of association until the cell is 
      virtually saturated with ligand. If the rate of reaction is not diffusion 
      limited, then the opposite holds; i.e., the forward velocities will be 
      proportional to the concentration of vacant receptors, but the reverse reactions 
      will not be. We examined the kinetics of reaction between immunoglobulin E (IgE) 
      and its receptor and clearly demonstrated that the reaction is not diffusion 
      controlled. The substantial (congruent to 30-fold) increase in the forward rate 
      constant observed for the reaction of IgE with solubilized receptors as opposed 
      to cell-bound receptors is therefore not an artifact of calculation. Since the 
      reverse rate constants show little difference, we postulate that the presence of 
      other surface components (rather than conformational differences in the receptor) 
      affects the reaction with the cells. As an aid to the analysis, the theory has 
      been extended so that not only the rate constants but also the entire course of 
      the reaction of ligand with cell receptors can be predicted for diffusion-limited 
      vs. non-diffusion-limited interactions.
FAU - Wank, S A
AU  - Wank SA
FAU - DeLisi, C
AU  - DeLisi C
FAU - Metzger, H
AU  - Metzger H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Ligands)
RN  - 0 (Receptors, IgE)
RN  - 0 (Receptors, Immunologic)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Basophils/immunology
MH  - Diffusion
MH  - Immunoglobulin E/*immunology
MH  - Kinetics
MH  - Leukemia, Experimental/immunology
MH  - Ligands
MH  - Mathematics
MH  - Rats
MH  - Receptors, IgE
MH  - Receptors, Immunologic/*immunology
EDAT- 1983/02/15 00:00
MHDA- 1983/02/15 00:01
CRDT- 1983/02/15 00:00
PHST- 1983/02/15 00:00 [pubmed]
PHST- 1983/02/15 00:01 [medline]
PHST- 1983/02/15 00:00 [entrez]
AID - 10.1021/bi00273a038 [doi]
PST - ppublish
SO  - Biochemistry. 1983 Feb 15;22(4):954-9. doi: 10.1021/bi00273a038.