PMID- 6223542
OWN - NLM
STAT- MEDLINE
DCOM- 19830826
LR  - 20190628
IS  - 0003-2697 (Print)
IS  - 0003-2697 (Linking)
VI  - 130
IP  - 2
DP  - 1983 Apr 15
TI  - Use of the airfuge for analysis and preparation of receptors incorporated into 
      liposomes: studies with the receptor for immunoglobulin E.
PG  - 514-20
AB  - A Beckman Airfuge has been employed for studying the interaction between lipids 
      and the receptor for immunoglobulin E (IgE). For analytic experiments, samples 
      were applied underneath a discontinuous sucrose gradient. After a 30-min 
      centrifugation in a fixed-angle rotor, liposomes floated toward the top of the 
      gradient whereas unincorporated receptor-IgE complexes remained at the bottom of 
      the tube. Liposomes with incorporated receptors were also efficiently separated 
      in the ACR-90 preparative rotor. These methods of "Airfuge flotation" can provide 
      useful adjuncts to more traditional methods for density-gradient centrifugation 
      especially when rapid analysis of small samples is desired.
FAU - Rivnay, B
AU  - Rivnay B
FAU - Metzger, H
AU  - Metzger H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Lipids)
RN  - 0 (Liposomes)
RN  - 0 (Receptors, IgE)
RN  - 0 (Receptors, Immunologic)
SB  - IM
MH  - Animals
MH  - Centrifugation, Density Gradient
MH  - Lipids/*analysis
MH  - Liposomes/*analysis
MH  - Rats
MH  - Receptors, IgE
MH  - Receptors, Immunologic/*isolation & purification
EDAT- 1983/04/15 00:00
MHDA- 1983/04/15 00:01
CRDT- 1983/04/15 00:00
PHST- 1983/04/15 00:00 [pubmed]
PHST- 1983/04/15 00:01 [medline]
PHST- 1983/04/15 00:00 [entrez]
AID - 0003-2697(83)90626-7 [pii]
AID - 10.1016/0003-2697(83)90626-7 [doi]
PST - ppublish
SO  - Anal Biochem. 1983 Apr 15;130(2):514-20. doi: 10.1016/0003-2697(83)90626-7.