PMID- 6223732
OWN - NLM
STAT- MEDLINE
DCOM- 19830923
LR  - 20181113
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 53
IP  - 1
DP  - 1983 Jul
TI  - Selective enhancement of human IgE production in vitro by synergy of pokeweed 
      mitogen and mercuric chloride.
PG  - 183-91
AB  - IgE production in vitro was investigated in cultures of human peripheral blood 
      mononuclear cells (MNC) from non-atopic donors with pokeweed mitogen (PWM), 
      mercuric chloride (HgCl2), or both. PWM alone induced a few IgE immunoglobulin 
      secreting cells (ISC) detected by reverse plaque forming cells (PFC) and many 
      IgG, IgM, and IgA PFC. HgCl2 alone failed to produce significant numbers of ISC 
      of any class. PWM plus HgCl2 caused a selective increase of IgE PFC without 
      affecting IgG, IgM, and IgA PFC. Co-cultures of B cells plus mitomycin C (MMC) 
      treated T cells stimulated by PWM alone produced more IgG, IgM, IgA and IgE PFC 
      than those of B cells plus T cells. However, PWM plus HgCl2 produced 
      significantly more IgE PFC selectively in those cultures. This effect was 
      observed in the cells of most of the donors, but a few donors showed different 
      responses.
FAU - Kimata, H
AU  - Kimata H
FAU - Shinomiya, K
AU  - Shinomiya K
FAU - Mikawa, H
AU  - Mikawa H
LA  - eng
PT  - Journal Article
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Pokeweed Mitogens)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 53GH7MZT1R (Mercuric Chloride)
RN  - FXS1BY2PGL (Mercury)
SB  - IM
MH  - Adult
MH  - Antibody-Producing Cells/drug effects
MH  - Cells, Cultured
MH  - Drug Synergism
MH  - Hemolytic Plaque Technique
MH  - Humans
MH  - Immunoglobulin E/*biosynthesis
MH  - Lymphocytes/drug effects/*immunology
MH  - Mercuric Chloride
MH  - Mercury/*pharmacology
MH  - Pokeweed Mitogens/*pharmacology
PMC - PMC1535527
EDAT- 1983/07/01 00:00
MHDA- 1983/07/01 00:01
PMCR- 1984/07/01
CRDT- 1983/07/01 00:00
PHST- 1983/07/01 00:00 [pubmed]
PHST- 1983/07/01 00:01 [medline]
PHST- 1983/07/01 00:00 [entrez]
PHST- 1984/07/01 00:00 [pmc-release]
PST - ppublish
SO  - Clin Exp Immunol. 1983 Jul;53(1):183-91.