PMID- 6229273
OWN - NLM
STAT- MEDLINE
DCOM- 19840323
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 22
IP  - 25
DP  - 1983 Dec 6
TI  - Covalent cross-linking of subunits of the receptor for immunoglobulin E induced 
      by immunoprecipitation.
PG  - 5729-32
AB  - The receptor on rat basophilic leukemia and related normal cells that binds 
      monomeric immunoglobulin E (IgE) with high affinity contains four polypeptide 
      chains: alpha (to which the IgE binds), beta, and a disulfide-linked dimer of 
      gamma chains. In this study, we have analyzed a further component variably seen 
      when the purified receptors are analyzed on polyacrylamide gels. This component 
      has an apparent Mr of approximately 43 000 and, after treatment with reducing 
      agents, yields one beta and two gamma chains. This complex is generated by 
      immunoprecipitation of preparations totally lacking in it. This novel in vitro 
      phenomenon has provided additional information about the structure of the 
      receptor. Its possible relationship to in vivo aggregation that triggers 
      degranulation of the cells is of interest.
FAU - Kinet, J P
AU  - Kinet JP
FAU - Perez-Montfort, R
AU  - Perez-Montfort R
FAU - Metzger, H
AU  - Metzger H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Macromolecular Substances)
RN  - 0 (Receptors, IgE)
RN  - 0 (Receptors, Immunologic)
SB  - IM
MH  - Animals
MH  - Basophils
MH  - Cell Line
MH  - Immunosorbent Techniques
MH  - Leukemia/metabolism
MH  - Macromolecular Substances
MH  - Mice
MH  - Molecular Weight
MH  - Rats
MH  - Receptors, IgE
MH  - Receptors, Immunologic/*metabolism
EDAT- 1983/12/06 00:00
MHDA- 1983/12/06 00:01
CRDT- 1983/12/06 00:00
PHST- 1983/12/06 00:00 [pubmed]
PHST- 1983/12/06 00:01 [medline]
PHST- 1983/12/06 00:00 [entrez]
AID - 10.1021/bi00294a008 [doi]
PST - ppublish
SO  - Biochemistry. 1983 Dec 6;22(25):5729-32. doi: 10.1021/bi00294a008.