PMID- 6229531
OWN - NLM
STAT- MEDLINE
DCOM- 19840305
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 259
IP  - 2
DP  - 1984 Jan 25
TI  - Reconstitution of the receptor for immunoglobulin E into liposomes. 
      Reincorporation of purified receptors.
PG  - 1212-7
AB  - Mast cells and related cells have on their surface receptors that bind 
      immunoglobulin E (IgE) with high affinity and which, when aggregated, trigger 
      exocytosis. We recently demonstrated that when these receptors are solubilized 
      with mild detergents, their subunits dissociate unless an appropriate 
      lipid:detergent ratio is maintained. The conditions required to maintain the 
      receptors' integrity appeared to parallel those previously determined as 
      necessary to obtain adequate incorporation of unpurified IgE-receptor complexes 
      from detergent extracts into liposomes. We now show that purified IgE-receptor 
      complexes having the full complement of subunits become preferentially inserted 
      into liposomes. If the receptor subunits are chemically cross-linked to each 
      other, at least some of such receptors can be incorporated, even though lipid is 
      omitted during their purification. The findings suggest that the IgE-binding 
      alpha subunit of the receptor is anchored to the bilayer by means of one or both 
      of the other subunits.
FAU - Rivnay, B
AU  - Rivnay B
FAU - Rossi, G
AU  - Rossi G
FAU - Henkart, M
AU  - Henkart M
FAU - Metzger, H
AU  - Metzger H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Liposomes)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Receptors, IgE)
RN  - 0 (Receptors, Immunologic)
SB  - IM
MH  - Animals
MH  - Centrifugation, Density Gradient
MH  - Cross-Linking Reagents/pharmacology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Liposomes/*metabolism
MH  - Macromolecular Substances
MH  - Mice
MH  - Rats
MH  - Receptors, IgE
MH  - Receptors, Immunologic/*metabolism
EDAT- 1984/01/25 00:00
MHDA- 1984/01/25 00:01
CRDT- 1984/01/25 00:00
PHST- 1984/01/25 00:00 [pubmed]
PHST- 1984/01/25 00:01 [medline]
PHST- 1984/01/25 00:00 [entrez]
AID - S0021-9258(17)43590-3 [pii]
PST - ppublish
SO  - J Biol Chem. 1984 Jan 25;259(2):1212-7.