PMID- 6235063
OWN - NLM
STAT- MEDLINE
DCOM- 19840919
LR  - 20190706
IS  - 0009-8981 (Print)
IS  - 0009-8981 (Linking)
VI  - 140
IP  - 1
DP  - 1984 Jun 27
TI  - Hexokinase, phosphofructokinase and pyruvate kinase isoenzymes in lymphocyte 
      subpopulations.
PG  - 65-76
AB  - In order to study the three regulator enzymes of glycolysis, hexokinase (HK), 
      phosphofructokinase (PFK) and pyruvate kinase (PK), in relation to lymphocyte 
      maturation, lymphocytes of different origin were investigated. Lymphocytes from 
      bone marrow, thymus, cord blood, adult peripheral blood and mitogen-stimulated 
      lymphocytes were investigated. The enzyme activities were determined and the 
      isozyme patterns were studied by means of electrophoresis, kinetic measurements 
      and immunoprecipitation. The young lymphocytes from bone marrow and the 
      mitogen-stimulated lymphocytes could be distinguished from the other lymphocytes 
      by a higher residual HK activity in the presence of the inhibitor 
      glucose-1,6-diphosphate. Peripheral blood T lymphocytes differed from non-T 
      lymphocytes in the PK isozymes distribution. All the cells contained PK type K4 
      and the hybrid K3M. In T cells a smaller amount of the K isozyme was seen than in 
      non-T cells. The PK residual activity in the presence of alanine was 
      significantly higher in peripheral blood T cells than in non-T cells. Thymocytes 
      are characterised by a larger amount of PFK M-subunits than peripheral blood T 
      and non-T lymphocytes. The stimulation of PFK by the positive effector 
      glucose-1,6-diphosphate was higher in thymocytes than in the peripheral blood 
      lymphocytes.
FAU - Kraaijenhagen, R J
AU  - Kraaijenhagen RJ
FAU - van der Heijden, M C
AU  - van der Heijden MC
FAU - Streefkerk, M
AU  - Streefkerk M
FAU - Rijksen, G
AU  - Rijksen G
FAU - de Gast, G C
AU  - de Gast GC
FAU - Staal, G E
AU  - Staal GE
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
RN  - 0 (Isoenzymes)
RN  - 0 (Mitogens)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.11 (Phosphofructokinase-1)
RN  - EC 2.7.1.40 (Pyruvate Kinase)
SB  - IM
MH  - Bone Marrow/enzymology
MH  - Electrophoresis, Cellulose Acetate
MH  - Fetal Blood/enzymology
MH  - Granulocytes/enzymology
MH  - Hexokinase/*analysis/antagonists & inhibitors/blood
MH  - Humans
MH  - Isoenzymes/analysis
MH  - Kinetics
MH  - Lymphocytes/classification/drug effects/*enzymology
MH  - Mitogens/pharmacology
MH  - Monocytes/enzymology
MH  - Phosphofructokinase-1/*analysis/antagonists & inhibitors/blood
MH  - Precipitin Tests
MH  - Pyruvate Kinase/*analysis/antagonists & inhibitors/blood
MH  - T-Lymphocytes/enzymology
MH  - Thymus Gland/cytology/enzymology
EDAT- 1984/06/27 00:00
MHDA- 1984/06/27 00:01
CRDT- 1984/06/27 00:00
PHST- 1984/06/27 00:00 [pubmed]
PHST- 1984/06/27 00:01 [medline]
PHST- 1984/06/27 00:00 [entrez]
AID - 0009-8981(84)90152-9 [pii]
AID - 10.1016/0009-8981(84)90152-9 [doi]
PST - ppublish
SO  - Clin Chim Acta. 1984 Jun 27;140(1):65-76. doi: 10.1016/0009-8981(84)90152-9.