PMID- 6235183
OWN - NLM
STAT- MEDLINE
DCOM- 19840917
LR  - 20190825
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 8
IP  - 1
DP  - 1984
TI  - Fractionation of mast cell components for studies of ligand-receptor binding at 
      the plasma membrane.
PG  - 43-7
AB  - This communication describes a simple procedure for fractionating mast cells 
      producing plasma membranes and intact granules. Mast cells were purified over a 
      bovine serum albumin density gradient and disrupted under conditions in which no 
      histamine was released. Iodinated immunoglobulin E (IgE) bound to the cells 
      served as a marker for the plasma membrane fraction. Employing a discontinuous 
      sucrose gradient the plasma membrane and granule fractions were separated. The 
      specific activity of the IgE binding to the isolated plasma membrane fractions 
      was 10-fold higher compared with that of the IgE binding to intact cells.
FAU - Sagi-Eisenberg, R
AU  - Sagi-Eisenberg R
FAU - Foreman, J C
AU  - Foreman JC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Receptors, IgE)
RN  - 0 (Receptors, Immunologic)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - *Cell Fractionation
MH  - Cell Membrane/*analysis
MH  - Centrifugation, Density Gradient
MH  - Immunoglobulin E/*metabolism
MH  - Male
MH  - Mast Cells/*analysis
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Receptors, IgE
MH  - Receptors, Immunologic/isolation & purification/*metabolism
MH  - Ultrasonics
EDAT- 1984/01/01 00:00
MHDA- 1984/01/01 00:01
CRDT- 1984/01/01 00:00
PHST- 1984/01/01 00:00 [pubmed]
PHST- 1984/01/01 00:01 [medline]
PHST- 1984/01/01 00:00 [entrez]
AID - 0165-2478(84)90103-2 [pii]
AID - 10.1016/0165-2478(84)90103-2 [doi]
PST - ppublish
SO  - Immunol Lett. 1984;8(1):43-7. doi: 10.1016/0165-2478(84)90103-2.