PMID- 6236227
OWN - NLM
STAT- MEDLINE
DCOM- 19841024
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 99
IP  - 3
DP  - 1984 Sep
TI  - Lateral electromigration and diffusion of Fc epsilon receptors on rat basophilic 
      leukemia cells: effects of IgE binding.
PG  - 778-87
AB  - We have used in situ electromigration and post-field relaxation (Poo, M.-m., 
      1981, Annu. Rev. Biophys. Bioeng., 10:245-276) to assess the effect of 
      immunoglobulin E (IgE) binding on the lateral mobility of IgE-Fc receptors in the 
      plasmalemma of rat basophilic leukemia (RBL) cells. Bound IgE sharply increased 
      the receptor's electrokinetic mobility, whereas removal of cell surface 
      neuraminic acids cut it to near zero. In contrast, we found only a small 
      difference between the lateral diffusion coefficients (D) of vacant and 
      IgE-occupied Fc receptors (D: 4 vs. 3 X 10(-10) cm2/s at 24 degrees C). This is 
      true for monomeric rat IgE; with mouse IgE, the difference in apparent diffusion 
      rates was slightly greater (D: 4.5 vs. 2.3 X 10(-10) cm2/s at 24 degrees C). This 
      range of D values is close to that found in previous photobleaching studies of 
      the IgE-Fc epsilon receptor complex in RBL cells and rat mast cells. Moreover, 
      enzymatic depletion of cell coat components did not measurably alter the 
      diffusion rate of IgE-occupied receptors. Thus, binding of fluorescent 
      macromolecular probes to cell surface proteins need not severely impede lateral 
      diffusion of the probed species. If the glycocalyx of RBL cells does limit 
      lateral diffusion of the Fc epsilon receptor, it must act primarily on the 
      receptor itself, rather than on receptor-bound IgE.
FAU - McCloskey, M A
AU  - McCloskey MA
FAU - Liu, Z Y
AU  - Liu ZY
FAU - Poo, M M
AU  - Poo MM
LA  - eng
GR  - GM 30666-02/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Receptors, Fc)
RN  - 0 (Receptors, IgE)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Basophils/immunology
MH  - Cell Line
MH  - Cell Membrane/immunology/physiology/ultrastructure
MH  - Diffusion
MH  - Fluorescent Antibody Technique
MH  - Glycosaminoglycans/analysis
MH  - Immunoglobulin E/*metabolism
MH  - Leukemia, Experimental/*immunology/metabolism
MH  - Microscopy, Electron
MH  - Rats
MH  - Receptors, Fc/*metabolism
MH  - Receptors, IgE
PMC - PMC2113395
EDAT- 1984/09/01 00:00
MHDA- 1984/09/01 00:01
PMCR- 1985/03/01
CRDT- 1984/09/01 00:00
PHST- 1984/09/01 00:00 [pubmed]
PHST- 1984/09/01 00:01 [medline]
PHST- 1984/09/01 00:00 [entrez]
PHST- 1985/03/01 00:00 [pmc-release]
AID - 84289681 [pii]
AID - 10.1083/jcb.99.3.778 [doi]
PST - ppublish
SO  - J Cell Biol. 1984 Sep;99(3):778-87. doi: 10.1083/jcb.99.3.778.